摘要
目的：研究了离子对反相高效液相色谱法测定小柴胡胶囊制剂中黄芩苷的含量。方法：用５０ ％ 甲醇作溶剂，超声波振荡法提取样品。色谱条件用μＢｏｎｄｐａｋ Ｃ１８ 作分析柱，Ｗａｔｅｒｓ ＧｕａｒｄＰＡＫ Ｃ１８ 作保护柱，水相（ 取庚烷磺酸钠０ ．４０ｇ ，磷酸二氢钠３ ．９０ｇ ，冰醋酸２０ｍｌ ，加水溶解并稀释至１０００ｍｌ）甲醇（５６∶４４） 作流动相，流速１ｍｌ／ｍｉｎ ，ＵＶ检测波长为２７５ｎｍ 。结果：黄芩苷与其它组份峰分离良好，柱效高，保留时间约为１２ｍｉｎ ，在浓度０ ．０３ ～０ ．２０ｍｇ／ｍｌ之间与峰面积（Ａ／１０７） 呈良好线性关系，回归方程为：Ａ＝ ０ ．００８ ＋ ５ ．６０８ｃ ，ｒ ＝ ０ ．９９９９６ ，５ 次的加样回收率为１００ ．２ ％ ，ＲＳＤ＝ ０ ．８４ ％ ，日内、日间变异（ ＲＳＤ） 分别为０ ．８６ ％ 和１ ．４２ ％ 。结论：本法简便、准确。
OBJECTIVE:To determine the content of baicalin in Xiaochaihu capsules by ion pair reversed phase high performance liquid chromatography.METHOD:The sample was extracted with 50ml of methanol water(1∶1) by ultrasonic wave vibration for 40 minutes.Baicalin was separated by μ Bondapk C 18 (10μm,300×3.9mm ID).The mobile phase was water(mix 0.40g sodium octanesulfonate,3.90g sodium dihydrogen phosphate and 20ml glacial acetic acid,and add water to 1000ml) methanol(56∶44) with sodium octanesulfonate as counterion.The detective wavelength was 275nm and the flow rate was 1ml/min.RESULTS:There was a good linear relationship in the concentration range of 30～200μg/ml with r= 0.99996 .The average recovery and the relative standard deviation was 100.2% and 0.84%(n=5),respectively.The relative standard deviation of within day and between day were 0.86% and 1.42%.CONCLUSION:.The method is simple,accurate,and can be suitable for the determination of baicalin in the preparation
出处
《中国现代应用药学》
CAS
CSCD
1999年第5期49-51,共3页
Chinese Journal of Modern Applied Pharmacy
