摘要
目的探讨部分门静脉动脉化重建肝血流后对肝内胆管和胆管周围血管丛的影响。方法 60只大鼠随机分为对照组、肝固有动脉结扎(HAL)组和HAL+部分门静脉动脉化(APS)组,动态观察术后3,7,30 d血清生化指标及肝内汇管区病理组织学变化,并行PCNA和TUNEL染色统计汇管区胆管细胞增殖和凋亡情况。结果 (1)3组血清生化值比较均无统计学差异(P>0.05)。(2)HAL组术后3d出现汇管区炎症反应,表现为汇管区炎细胞浸润、小叶间门静脉管壁增厚和胆管细胞退行性变,术后1个月后汇管区炎症反应基本恢复正常,无胆小管反应。APS组汇管区炎症反应较HAL组明显减轻,未见明显胆管细胞的退行性改变。(3)3组汇管区微血管计数均无统计学差异(P>0.05);3组大鼠肝组织TUNEL原位凋亡检测均未见阳性胆管细胞;3组汇管区胆管细胞核增殖指数无统计学差异(P>0.05)。结论部分门静脉动脉化可以改善肝固有动脉结扎后早期汇管区的缺血性炎症反应,并可促进肝损害的早期恢复。
Objective To investigate the effect of arterio-portal shunt（APS） on intrahepatic bile duct and peribiliary plexus（PBP）.Methods Sixty rats were divided into three groups： SHAM group,hepatic artery ligation（HAL） group and HAL＋APS group.Serum biochemical tests,histological and immunohistochemical analyses were performed to evaluate the pathological changes in portal area on d1,d7 and d30.Results（1）The serum biochemical test showed no significant difference between the three groups at 3 time points.（2） HAL induced inflammatory response in portal area,which was characterized by infiltration of inflammatory cells,thickening of the wall of interlobular portal vein and degenerative changes of cholangiocytes,but the pathological changes returned to normal by day 30.The APS group showed a transient inflammatory response which was milder than that of the HAL group.（3）Microvessel count,apoptosis index and PCNA index of cholangiocyte showed no significant difference between the three groups.Conclusions APS can improve portal area inflammation caused by HAL and promote early recovery of liver damage.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2011年第2期150-153,共4页
China Journal of General Surgery
关键词
胆管
肝内
门静脉动脉化
细胞增生
细胞凋亡
Bile Ducts
Intrahepatic
Portal Vein Arterlizations
Cell Proliferation
Apoptosis
