摘要
目的研究局部短时间应用高浓度蒸馏水顺铂溶液对骨肉瘤细胞的杀伤作用。方法培养骨肉瘤细胞U2-OS。按照作用于骨肉瘤细胞药物的不同,分为蒸馏水组(DW组),质量浓度分别为31.25(DW31.25组)、62.5(DW62.5组)、125(DW125组)、250(DW250组)、500μg/mL(DW500组)的蒸馏水顺铂组。培养的骨肉瘤细胞按照分组给予药物作用5min。于0、24、48、72、96h,用MTT法测定肿瘤细胞抑制率,进行统计学分析,并用倒置显微镜观察细胞数量和形态变化。结果在0h时,各组肿瘤细胞抑制率之间无显著性差异(P>0.05)。随着时间的延长,在96h时,DW62.5组、DW125组、DW250组和DW500组肿瘤细胞抑制率之间无显著性差异(P>0.05),均高于DW31.25组和DW组,且有显著性差异(P<0.05)。在96h时,DW62.5组、DW125组、DW250组和DW500组几乎没有骨肉瘤细胞存活生长,而DW组仍有较多骨肉瘤细胞存活生长。结论局部短时间应用高质量浓度蒸馏水顺铂溶液对骨肉瘤细胞有强大的杀伤作用。
Objective：To study the effects of high-concentration distilled water（DW） and cisplatin administered locally in a short time on osteosarcoma（OS） cells.Methods：Cultured osteosarcoma cells,U2-OS,were divided into the DW group,the DW31.25 group,the DW62.5 group,the DW125 group,the DW250 group and the DW500 group according to the corresponding concentrations of cisplatin applied,i.e.31.25 μg/mL,62.5 μg/mL,125 μg/mL,250 μg/mL and 500 μg/mL,respectively.Then,osteosarcoma cells were processed for 5 minutes.At 0,24,48,72 and 96h,the inhibition rates of tumor cells were determined by MTT assay.Changes of cells in morphology and quantity were observed under inverted microscopy.Results：Among the groups,there was no significant difference in the inhibition rates at 0 h.Though no differences were observed among the DW62.5 group,the DW125 group,the DW250 group and the DW500 group at 96h,the inhibition rates were significantly higher than those of the DW group and the DW31.25 group.In the former 4 groups,hardly any osteosarcoma cells survived at 96h while massive osteosarcoma cells survived in the DW group.Conclusion：High-concentration distilled water and cisplatin administered in a local and short-time manner produces strong killing effect on osteosarcoma cells.
出处
《山东大学学报（医学版）》
CAS
北大核心
2011年第2期75-78,共4页
Journal of Shandong University:Health Sciences
关键词
骨肉瘤
顺铂
蒸馏水
Osteosarcoma
Cisplatin
Distilled water
