摘要
探索大豆中胰蛋白酶抑制剂有效、快速的钝化方法。通过正交试验设计研究温度、时间、亚硫酸钠浓度对抑制因子的钝化影响,采用酚红法和国家标准法(GB/T8622-2006)对钝化后大豆中脲酶活性进行定性、定量检测。最优钝化工艺条件为:温度75℃、时间60 min、Na2SO3浓度0.03 mol.L-1。亚硫酸钠法是一种简便、实用的大豆胰蛋白酶抑制剂钝化方法。
Objective： To explore the effective and rapid passivation of the anti-nutritional factors in soybean.Methods： The passivation conditions were optimized with orthogonal test design concerning temperature,time and sodium sulfite concentration.Determination of urease activity in soybean after passivation with red phenol assay and GB/T 8622-2006 assay.Results： The optimal technological parameters：passivate temperature was 75 ℃,passivate time was 60 min and sodium sulfite concentration was 0.03 mol·L-1.Conclusion： The sodium sulfite method is a feasible and effective passivation treatment.
出处
《黑龙江八一农垦大学学报》
2011年第1期31-33,共3页
journal of heilongjiang bayi agricultural university
基金
黑龙江农垦总局课题项目(HNKXIV-02-07a)
关键词
亚硫酸钠
胰蛋白酶抑制剂
钝化
脲酶活性
sodium sulfite
soybean trypsin inhibitor
passivate
urease activity
