摘要
利用高效液相色谱法同时分析测定了血液中吗啡、杜冷丁、安定的质量浓度。分析柱为C18，流动相为V（甲醇）：V（25mmol／LKH2PO4）＝90：10，检测波长为285nm。样品血液pH8．5～9．4时，用V（氯仿）：V（异丙醇）＝9：1溶液提取，以氮气吹干有机溶剂后用流动相溶解残渣进样分析。线性范围为0．05～50mg／L，最小检出质量浓度为0．05mg／L，日内与日间精密度CV＜6％。
Morphine, penthidine and diazepam in blood were determined by high performance liquid chromatography(HPLC). The HPLC system was consisted of 10 μm particle size C18 column and UV detector set at 254 um. The mobile phase was V(CH3OH): V(25 mmol/L KH2PO4) = 90: 10 with a flow rate of 1. 0mL/min. The average recoveries of morphine, penthidine and diazepam were 93. 27%, 90. 23% and 93. 29%respectively. The linear range was between 0. 05-50 mg/L. The detection limit was 0. 05 mg/L and CV was less than 6 %.
出处
《色谱》
CAS
CSCD
北大核心
1999年第4期399-400,共2页
Chinese Journal of Chromatography
关键词
吗啡
杜冷丁
安定
血药浓度
HPLC
测定
high performance liquid chromatography
morphine
penthidine
diazepam
blood mass concentration
