摘要
目的:探讨β1-整合素和Shh在不同分级、分期大肠癌的表达情况及其相互关系。方法:采用免疫组化SP法,检测β1-整合素和Shh在大肠癌组织芯片中的表达情况,并进行二者的相关性分析。结果:大肠癌组织中β1-整合素和Shh蛋白的表达阳性率分别为59.42%、71.01%。β1-整合素的阳性表达与大肠癌的浸润深度、Ducks分期、淋巴结转移有关(P<0.05),而与性别、年龄、分化程度的相关性均无统计学意义。Shh的阳性表达仅与大肠癌的分化程度有关。β1-整合素和Shh在大肠癌表达存在相关性(r=0.326,P<0.05)。结论:β1-整合素的阳性表达与大肠癌的浸润深度、Ducks分期、淋巴结转移有关,大肠癌中β1-整合素和Shh的表达存在等级相关,联合检测二者在大肠癌的表达,可作为大肠癌恶性程度评价的一个较有价值的指标,并将有助于深入认识肿瘤的生物学基础和寻找有效的治疗药物。
Objective： To investigate the expression of β1-integrin and Shh in colorectal cancer and their relationship.Methods： Immunohistochemical method of SP was used to exam β1-integrin and Shh expression in the cases of colorectal cancer,moreover,the correlation between them were analyzed.Results： In colorectal cancer,positive expression rate of β1-integrin was 59.42%,and the positive expression rate of Shh was 71.01%.Significant differences in the expression of β1-integrin was found in different depth of invasion,lymph node metastasis and TNM staging（all P0.05）.No significant differences in the expression of β1-integrin was found in different gender,age and pathological grading,while significant difference in the expression of Shh was only found in different pathological grading（P0.05）.A positive correlation was found between the expression of β1-integrin and Shh（P0.05）.Conclusion： The positive expression of β1-integrin was related to the depth of invasion,lymph node metastasis and TNM staging,and the expression of Shh and β1-integrin in colorectal cancer was correlated.The combined detection of β1-integrin and Shh may be of important clinical value for evaluating the degree of malignancy of colorectal cancer as well as better understanding the biological basis of cancer and finding effective treatments.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2011年第2期249-252,I0004,共5页
Medical Journal of Wuhan University
