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黄连吴茱萸配伍对大鼠高脂血症模型肝脏组织ABCA_1基因表达的影响 被引量：14

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摘要 目的:考察黄连、吴茱萸等比配伍对大鼠高脂血症模型肝脏ABCA1基因表达的影响。方法:高脂乳剂建立大鼠高脂血症模型,采用免疫透射比浊法测定血清apoA1含量,以18sRNA为内参基因,实时荧光定量PCR技术检测肝组织中ABCA1 mRNA相对表达水平;结果:高脂血症模型血清apoA1含量较正常组显著降低,黄连、吴茱萸等比配伍粉剂能够显著提高高脂血症模型大鼠血清中apoA1含量;模型组ABCA1 mRNA较正常组显著低表达(P<0.05),各给药组ABCA1 mRNA均较模型组表达显著增高,其中总碱配伍高剂量组提高最为显著(P<0.01),总碱配伍低剂量组ABCA1 mRNA表达增高倍数最低,粉剂组较水煎液组ABCA1 mRNA表达高;结论:黄连、吴茱萸等比配伍可以提高大鼠高脂血症模型肝组织ABCA1 mRNA的表达,提示黄连吴茱萸配伍增强肝组织表达ABCA1 mRNA,促进胆固醇逆向转运是其发挥降血脂作用的主要环节之一。
作者 沈涛 贾波 郭力 徐世军
机构地区 成都中医药大学
出处 《成都中医药大学学报》 2011年第1期49-51,共3页 Journal of Chengdu University of Traditional Chinese Medicine
基金 四川省中医药管理局重点项目(编号:200620) 四川省科技厅应用基础研究项目(编号:07JY029-092)
关键词 黄连吴茱萸配伍 ABCA1基因 降血脂 机理
分类号 R289.5 [医药卫生—方剂学] R589.2 [医药卫生—内分泌]
  • 相关文献

参考文献10

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  • 7刘胜林,郭志刚,刘凌,李欣.ABCA1基因转录调节与胆固醇代谢及动脉粥样硬化的关系[J].中国分子心脏病学杂志,2004,4(5):308-312. 被引量：7
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二级参考文献57

  • 1[1]Santamaina-fojo S, Pterson K, Knapper C, et al. Complete genomic sequence of the human ABCA1: analysis of the human and mouse ATP binding cassette promoter A. Proc Natl Acad Sci, USA, 2000, 97 (14): 7987-7992
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  • 3[3]Reddy ST, Hama S, Ng C, et al. ATP-binding cassette transporter 1 participates in LDL oxidation by artery wall cells.Arterioscler Thromb Vasc Biol, 2002, 22 (11 ): 1877-1883
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  • 8[8]Huuskonen J, Vishnu M, Pullinger CR, et al. Regulation of ATP-binding cassette transporter A1 transcription by thyroid hormone receptor. Biochemistry, 2004, 43 (6): 1626-1632
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共引文献27

同被引文献247

引证文献14

二级引证文献103

成都中医药大学学报
2011年 第1期

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