摘要
目的:研究采用阳离子交换树脂法富集延胡索生物碱的工艺参数。方法:以总生物碱交换量为考察指标,经阳离子交换树脂法富集后,采用紫外分光光度法测定。结果:纯化工艺条件为药材-浓缩液=1∶5做为上柱液,药材比树脂为1∶1,流速2 BV.h-1,径高比约为1∶10,5 BV的纯化水2 BV.h-1水洗除杂,4 BV的含5%氨水的70%乙醇2 BV.h-1洗脱树脂柱。结论:该法富集纯化生物碱稳定可行。
Objective： To study the method of enrichment of fumaric alkaloids by using cation resin.Method： Ultraviolet spectrophotometry was used to determine total fumaric alkaloid with exchange capacity as index.Result： The optimum purification condition was as follows： herbs： concentrate solution was about 1 ∶ 5,herbs： cation resin was about 1∶ 1;flow rate was 2 BV.h-1,diameter：high of cation resin column was about 1∶ 10;5 BV of purified water eluted resin column to removed impurities,flow rate was 2 BV.h-1,4 BV of 70% ethanol as eluent consisted of 5% ammonia,2 BV.h-1 flow rate washing resin column.Conclusion： The optimum condition was stable and can be used for purification of alkaloids.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第7期40-43,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
延胡索
树脂
紫外-可见分光光度法
分离纯化
Corgdalis Rhizoma
resins
ultraviolet spectrophotometry
separation and purification
