摘要
目的:建立活血养阴颗粒的质量标准。方法:采用薄层色谱法对活血养阴颗粒中的葛根、益母草进行定性鉴别;采用HPLC对制剂中葛根素进行了含量测定,高效液相色谱条件Alltima C18柱(4.6 mm×250 mm,5μm),流动相甲醇-0.3%磷酸溶液(26∶74),流速1.0 mL.min-1,检测波长250 nm,柱温35℃,以外标法计算含量。结果:TLC色谱中能检出葛根、益母草;高效液相色谱测定葛根素在0.041 8～1.002 2μg呈良好的线性关系,r=0.999 9,平均回收率99.4%,RSD 1.4%(n=6)。结论:该法可准确地进行定性、定量,可有效地控制活血养阴颗粒的质量。
Objective：To establish the quality standard of Huoxue Yangyin granules composed of Herb Leonuri,Radix Puerariae Lobatae.Method： The quality of each raw material was identified by TLC.The quantity of the preparation was conducted by HPLC and the method was developed with the condition as follows： Alltima C18（4.6 mm × 250 mm,5 μm）,mobile phase methanol-0.3% phosphoric acid（26∶ 74）,the temperature of column 35 ℃,flow rate 1.0 mL.min-1,calculating the content with external standard method.Result： Herb Leonuri,Radix Puerariae Lobatae could be detected by TLC.Puerarin was linear in a range of 0.041 8 ～ 1.002 2 μg（r =0.999 9）.The average recovery was 99.4%,and RSD was 1.4%（n = 6）.Conclusion： This method is simple,reliable,accurate,and can be used to control the quality of Huoxue Yangyin granules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第7期65-67,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏省社发项目(BS2007071)
