摘要
本文利用激光扫描共聚焦显微镜（LSCM）对促甲状腺素（TSH）及白细胞介素-6（IL6）诱导的FRTL－5细胞内Ca2+变化进行了研究。方法：培养的FRTL－5细胞以FLuo－3染色，用LSCM在488nm扫描并加入不同浓度的TSH及IL－6检测细胞内Ca2+的变化。结果：TSH（1－5U／L）可使细胞内Ca2+的水平上升，且与TSH的浓度有一定关系。IL－6（1～100μg／L）对FRTL－5细胞内基础Ca+浓度无直接作用。
The intracellular Ca2+ level ([Ca2+]i) of rat thyroid FRTL-5 cells was studied by laser scanning confocalmicroscope (LSCM). The Fluo-3 loaded FRTL-5 cells were exposed to the different concentrations of TSH and IL-6,and their [Ca2+]i was determined by LSCM. Results indicated that [Ca2+]i levels of FRTL-5 cells were increased after TSH,the maximal effect of TSH appeared at a concentration of SU/L; while IL-6 did not affect the basal levels of [Ca2+]i inFRTL-5 cells.
出处
《基础医学与临床》
CSCD
1999年第4期56-58,共3页
Basic and Clinical Medicine
关键词
白细胞介素6
甲状腺细胞
促甲状腺素
钙离子
laser scanning congocal microscope thyroid cells thyrotropin intracellular calcium
