摘要
目的检测非小细胞肺癌患者的表皮生长因子受体(EGFR)基因外显子的突变情况。方法收集24例肺腺癌和25例肺鳞癌手术标本,抽提组织DNA,对EGFR基因的1-28号外显子进行扩增并测序,筛查EGFR基因的突变。结果在8例肺腺癌患者中检出EGFR突变,在肺鳞癌患者中未检测到突变。检测到的突变位点位于19-21号外显子内,且均为非吸烟患者,占非吸烟肺腺癌患者的61.5%(8/13)。结论 EGFR基因突变在非吸烟的肺腺癌患者具有较高的发生率,突变主要集中发生于19-21号外显子。
Objective To detect the mutation condition of epidermal growth factor receptor（EGFR） in non-small cell lung cancer（NSCLC） patients.Methods Twenty-four adenocarcinoma（AC） of lung samples and twenty-five squamous cell carcinoma（SCC） samples were enrolled.DNA was extracted,and then the 1-28 exons of EGFR were amplified and sequenced to screen mutations.Results EGFR mutations were detected in eight patients with AC,however,there were no mutations in SCC patients.The mutations were all located within exons 19-21.All the patients who had EGFR mutations were non-smokers accounting 61.5%（8/13）of non-smoking AC patients.Conclusion EGFR activating mutations have high incidence rate in non-smoking AC patients.The EGFR mutations mainly locate within exons 19-21.
出处
《新乡医学院学报》
CAS
2011年第2期190-192,共3页
Journal of Xinxiang Medical University
关键词
非小细胞肺癌
表皮生长因子受体
基因突变
non-small cell lung cancer； epidermal growth factor receptor； genetic mutation
