摘要
目的分析不同剂量60Coγ射线部分照射人离体血对淋巴细胞染色体畸变形成的影响。方法用0～8Gy（剂量率为0．35Gy／min）60Coγ射线在37℃条件下照射3份离体健康人外周血标本，以0．5：1的比例与同一供血者的未受照血混合，120min后进行培养、制片，显微镜下分析染色体畸变（双着丝粒＋着丝粒环）的变化，借此进行剂量估算。结果各组的双着丝粒体＋着丝粒环和总畸变，以及断片和单体断裂均随着剂量的增加而增加。用双着丝粒＋着丝粒环进行的剂量估算，0．5—2Gy组大于照射剂量的1／3，4～8Gy组均接近照射剂量的1／3。结论染色体畸变可以作为估算非均匀照射的生物学指标之一。
Objective To investigate the effects of 60Coγ-ray partial radiation on chromosome aberration in human peripheral blood in vitro. Methods The samples of heparinized peripheral whole blood from 3 healthy persons were exposed to 60Coγ-rays at the doses between 0 and 8 Gy with the dose rate of 0. 35 Gy/min at the temperature of 37℃ , and then mixed with the unirradiated blood samples of the same donors by the ratio of 0.5： 1. 120 minutes later the samples of mixed blood underwent routine culture. Microscopy was used to observe the chromosome aberration double （ centromere ＋ centromere） and the biological dose was estimated thereby. Results The amounts of double centromere ＋ centromere were increased along with the dose of irradiation in all groups. The estimated biological dose was higher than the 1/3 of the irradiation dose when the dose was between 0.5 to 2 Gy, and was close to the 1/3 of the irradiation dose when the dose was between 4 to 8 Gy. Conclusion Chromosome aberration can be used as a biomarker in estimation of uneven irradiation.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2011年第1期38-40,共3页
Chinese Journal of Radiological Medicine and Protection
关键词
部分照射
人外周血
染色体畸变
Partial irradiation
Human peripheral blood
Chromosome aberration
