摘要
对影响杏离体繁殖的有关因素进行了研究。结果表明，杏的增殖和生长适宜的ＢＡ和ＩＢＡ质量浓度分别为１．０～１．５和０．０５～０．１ｍｇ·Ｌ－１，生根适宜的ＩＢＡ质量浓度为０．２ｍｇ·Ｌ－１．培养基中加入ＧＡ３和降低ＮＨ４ＮＯ３浓度有利于加长生长。随着继代培养次数的增加，芽的增殖、加长生长和生根效应均呈上升趋势。山梨醇作碳源时芽的增殖倍数高于蔗糖，而新梢生长差，生根率低。
Some factors influencing in vitro propagation of apricot (Armeniaca vulgaris Lam.) were studied.The results showed that the optimum concentrations of benzyladenine(BA) and indole 3 butyric(IBA) for shoot proliferation and growth were 1.0-1.5 and 0.05-0.1 mg/L,respectively.IBA at 0.2 mg/L was most favourable for rooting.Adding gibberellic acid(GA 3) and reducing NH 4NO 3 concentration of MS medium promoted shoot elongation.The proliferation rate of buds,shoot elongation and rooting ability had an increasing trend with increasing of subculture number.Sorbitol as carbon source promoted proliferation,but decreased shoot growth and rooting ability compared with sucrose.
出处
《西北农业大学学报》
CSCD
北大核心
1999年第4期12-15,共4页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
基金
陕西省自然科学基金
杨陵农业科技开发基金
关键词
杏
离体繁殖
增殖
加长生长
生根
apricot
in vitro propagation
proliferation
shoot elongation
rooting
