摘要
目的:了解临床标本中致病性大肠埃希菌的分布,研究从临床标本中分离致病性大肠埃希菌的方法。方法:采用多管PCR技术对1429份临床标本进行致病性大肠埃希菌的11种不同致病基因组进扩增,基因组检测阳性者再进行山梨醇麦康凯培养基分离培养和血清学凝集试验。结果:1429份临床标本中共检测出27株致病性大肠埃希菌,检出率为1.88%,分属于6个不同组。结论:本研究建立的多管PCR技术结合山梨醇麦康凯培养及血清学凝集方法,可敏感、快速地检测致病性大肠埃希菌。
Objective： To investigate the distribution of Shiga Toxin-producing Escherichia coil （STEC） from clinical specimen, and to explore a reliable method to detect STEC in clinical specimen. Methods：The Multi-tube PCR was used in Shiga toxin genome detection, SMAC medium and serum agglutination tests were used in identification of STEC. Results： 27 （1. 88~） of Shiga toxin genome positive strains were detected from 1429 specimen by Multi-tube PCR, the SMAC and serum agglutination tests verified that these 27 strains belong to 5 serum groups respectively. Conclusion：The improved Multi-tube PCR combined with the SMAC medium and serum agglutination tests technique can be widely used in screen STEC in clinical specimen.
关键词
致病性大肠埃希菌
多管PCR
检测
Shiga toxin-producing Escherichia coli
STEC Multi-tube PCR
Examination
