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黄瓜种子萌发对NO_3^-胁迫的响应及耐盐性评价 被引量:12

Response and Stress Tolerance Evaluation of Different Cucumber Cultivars under NO_3^- Stress
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摘要 通过培养皿发芽试验,研究了0、14、98、182 mmol/L NO3-对7个黄瓜品种种子萌发的影响,并用隶属函数法对它们的耐盐性进行综合评价.结果表明:与对照相比较,14 mmol/L NO3-处理使7个黄瓜品种种子的相对发芽率、相对发芽指数、耐盐指数9、6 h后的鲜重、干重和超微弱发光(UWL)强度均增加,而98、182 mmol/L NO3-处理下这些指标均逐渐降低;各幼苗的电解质相对渗漏率在14 mmol/L NO3-处理后降低,而在98、182 mmol/LNO3-处理后其值逐渐增加;随着NO3-浓度的增加,各黄瓜品种的胚根总面积、总长度逐渐减少.各黄瓜品种半致死浓度值(LC50)以津园5号的最大(192.3 mmol/L),新秀4号的最小(105.0 mmol/L).不同黄瓜品种耐盐性的综合评价显示,津园5号、新泰密刺为耐盐品种,新秀4号、神农春五为盐敏感品种,其他为中度耐盐品种. The effects of 0,14,98,and 182 mmol/L NO-3 on germination of 7 different cucumber cultivars in Petri dishes were studied and the NO-3 stress tolerance of different cucumber cultivars was evaluated.The results indicated that the relative germination rate,relative germination index,salt stress index,fresh weight,dry weight and ultraweak luminescence(UWL) intensity were increased after 14 mmol/L NO-3 treatment,but decreased after 98 and 182 mmol/L NO-3 treatments.The relative electrolyte leakage decreased after 14 mmol/L NO-3 treatment,but increased with 98 and 182 mmol/L NO-3 treatments.The total area and total length of radicals were decreased with the increase of NO-3 concentration.The value of LC50 of 'Jinyuan No.5' and 'Xinxiu No.4' were 192.3 mmol/L,105.0 mmol/L,respectively.In all of the 7 cultivars,'Jinyuan No.5' and 'Xintaimici' were salt tolerant cultivars,while 'Xinxiu No.4' and 'Shennongchunwu' were salt sensitive cultivars and others were intermediate salt tolerance by subordinate function values analysis.
出处 《西北植物学报》 CAS CSCD 北大核心 2011年第2期325-331,共7页 Acta Botanica Boreali-Occidentalia Sinica
基金 国家自然科学基金(30471187)
关键词 黄瓜 NO3-胁迫 超微弱发光 LC50 隶属函数 cucumber NO-3 stress ultraweak luminescence LC50 subordinate function
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