摘要
目的探讨阻塞性睡眠呼吸暂停综合征(OSAS)患者外周血白细胞端粒长度的变化及意义。方法 OSAS患者11例,健康对照组10例,提取外周血白细胞DNA,纯度检测合格后,用Q-PCR法检测端粒长度相对T/S比率。结果 OSAS组外周血白细胞端粒长度相对T/S比率较对照组小(P<0.05)。结论 OSAS患者外周血白细胞端粒长度的缩短可能与其发病机制有关,低氧血症和高碳酸血症加速了端粒的损耗和细胞的凋亡。
Objective To study the alteration of telomere length of the peripheral white blood cells in patients with obstructive sleep apnea syndrome（OSAS） and explore its significance.Methods The DNA was extracted from the peripheral white blood cells of 11 patients with OSAS and 10 normal subjects matched for age and gender,and the T/S ratio was measured by fluorescence quantitative PCR.Results The T/S ratio in the peripheral white blood cells of patients with OSAS was obviously lower than that of the normal subjects（P0.05）.Conclusion The reduction in the telomere length in the peripheral blood cells suggests a possible relationship between OSAS pathogenesis and telomere length,and hypoxemia and hypercapnia make accelerate telomere shortening and promote cell apoptosis.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2011年第3期457-460,共4页
Journal of Southern Medical University
基金
国家自然科学基金(39971789)
广东省自然科学基金(04020458)~~
