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体外循环与非体外循环冠状动脉旁路移植术循环内皮细胞损伤的研究 被引量:2

Circulating endothelial cell injury in on-pump and off-pump coronary-artery bypass grafting
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摘要 目的通过研究循环内皮细胞(CECs)数量和形态的改变,探讨体外与非体外冠状动脉旁路移植术中CECs的损伤有无差异。方法将48例需行冠状动脉旁路移植术的冠状粥样硬化疾病患者随机分为两组,分别接受体外与非体外循环下的手术治疗;在麻醉前、手术结束时、术后1d、3 d抽血,采用标记特异性抗体CD146的免疫磁珠技术分离CECs并计数;同时应用单细胞凝胶电泳技术观察CECs的DNA损伤程度。结果体外循环组CECs数量在手术结束及术后1天均显著高于非体外循环组(P<0.05),在术后3d,CECs数量两组无显著性差异(P>0.05);手术结束时体外循环组"彗尾"长度明显增长,荧光强度变大。结论体外循环较非体外循环冠状动脉旁路移植术相比,循环内皮细胞损伤严重;这些变化和预后有密切关系。 Objective To investigate the difference in circulating endothelial cell(CECs) injuries following on-pump and off-pump coronary-artery bypass surgery.Methods We randomly assigned 48 patients scheduled for urgent or elective coronary artery bypass grafting into two groups to receive on-pump and off-pump procedures.Blood samples were obtained before anesthesia,at the end of operation,and on days 1 and 3 after the operation,and CECs were isolated and counted using dynabeads coated with the specific antibody of CD146.Single cell gel electrophoresis was used to observe the morphological changes of the CECs.Results In the cardiopulmonary bypass(CPB) group,the number of CECs was significantly greater than that in non-CPB group(P0.05) at the end of surgery and 1 day after the operation.On postoperative day 3,the number CECs was similar between the two groups(P0.05).The length of the comet tail was longer in CPB group with stronger fluorescence intensity than in the non-CPB group.Conclusion Compared with of-pump coronary artery bypass grafting,on-pump coronary artery bypass grafting results in more serious CEC injury,which is closely related to the prognosis.
出处 《南方医科大学学报》 CAS CSCD 北大核心 2011年第3期535-538,共4页 Journal of Southern Medical University
关键词 血管内皮细胞 冠心病 体外循环 circulating endothelial cells coronary heart disease cardiopulmonary bypass
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  • 1Lee HZ, Yeh FT, Wu CH. The effect of elevated extracellular glucose on adherens junction proteins in cultured rat heart endothelial cells[J]. Life Sci, 2004, 74(17): 2085-96.
  • 2Quilici J, Banzet N, Paule P, et al. Circulating endothelial cell count as a diagnostic marker for non-ST-elevation acute coronary syndromes[J]. Circulation, 2004, 110(12): 1586-91.
  • 3王春玲,李宏伟,傅攀峰,张抒杨,高润霖,修瑞娟.冠心病患者循环内皮细胞与炎性相关因子的研究[J].中华心血管病杂志,2005,33(7):631-635. 被引量:17
  • 4Shroyer AL, Grover FL, Hattler B, et al. On-pump versus off-pump coronary-artery bypass surgery[J]. N Engl J Med, 2009, 361(19): 1827-37.
  • 5李宏伟,王春玲,修瑞娟.人循环内皮细胞的分离和鉴定[J].基础医学与临床,2006,26(6):640-643. 被引量:12
  • 6赵海峰,李学敏,李秀花,田志强,肖荣.神经管畸形DNA损伤单细胞凝胶电泳检测[J].中国公共卫生,2008,24(11):1352-1353. 被引量:2
  • 7谢俊刚,徐永健,张珍祥,张宁,倪望,陈仕新.DNA损伤与慢性阻塞性肺疾病的关系[J].中华内科杂志,2005,44(9):656-658. 被引量:2
  • 8Woywodt A, Streiber F, de Groot K, et al. Circulating endothelial cells as markers for ANCA-associated small-vessel vasculitis [J]. Lancet, 2003, 361(9353): 206-10.
  • 9Makin A J, Blann AD, Chung NA, et al. Assessment of endothelial damage in atherosclerotic vascular disease by quantification of circulating endothelial cells. Relationship with yon Willebrand factor and tissue factor[J]. Eur Heart J, 2004, 25(5): 371-6.
  • 10龙村,李景文,杨天宇,李庶,梁风玲,吉冰洋.体外循环对内皮素和循环内皮细胞的影响[J].中国循环杂志,1998,13(2):99-101. 被引量:7

二级参考文献34

  • 1孙亦阳,杨兆芬,谢继锋,沈业寿.采用SCGE和SCE分析法研究姬松茸菌体多糖(Ab-Mp)对DNA损伤的抑制作用[J].生物学杂志,2006,23(5):34-37. 被引量:3
  • 2刘强,姜恩海,李进,唐卫生,王知权,赵永成,樊飞跃.单细胞凝胶电泳技术对离体和整体照射致细胞DNA断裂的一致性研究[J].中国辐射卫生,2006,15(2):153-155. 被引量:13
  • 3杨映波,王正国,刘怀琼,刘国建,周蓉.血循环中内皮细胞数量变化意义的初探[J].第三军医大学学报,1990,12(3):209-211. 被引量:41
  • 4Thayer JM, Mirkes PE. Programmed cell death and n - acetoxy - 2 - acetylaminofluorene- induced apoptosis in the rat embryo[J ]. Tera Jology, 1995, 51:418 - 429.
  • 5Ejchart A, Sadlej - Sosnowska N. Statistical evaluation and comparison of comet assay rasults[J ]. Murat Res, 2003, 534 ( 1 - 20) : 85 - 92.
  • 6Olive PL, Wlode kD, Banatb JE. DNA double - strand breaks measured in individual cell subjected to gel electorophoresis[J]. Cancer Res, 1991, 51(17) :4671 - 4677.
  • 7Wang X F, Xing M L, Shen Y, et al. Oral administration of Cr (VI) induced oxidative stress, DNA damage and apoptotic cell death in mice[J]. Toxicology, 2006, 228 (1) :16 - 23.
  • 8Woywodt A, Streiber F, de Groot K, et al. Circulating endothelial cells as markers for ANCA-associated small-vessel vasculitis. Lancet, 2003,361:206-210.
  • 9Bull TM, Golpon H, Hebbel RP, et al. Circulating endothelial cells in pulmonary hypertension. Thromb Haemost, 2003, 90:698-703.
  • 10Engstrom G, Lind P, Hedblad B, et al. Effects of cholesterol and inflammation-sensitive plasma proteins on incidence of myocardial infarction and stroke in men. Circulation, 2002,105: 2632-2637.

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