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胰腺超声内镜细针穿刺活检物中K—ras基因定量检测对胰腺癌诊断价值的研究 被引量:6

Quantitative detection of K-ras gene mutation in samples from endoscopic ultrasound-guided fineneedle aspiration for pancreatic cancer
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摘要 目的比较不同胰腺超声内镜细针穿刺物中K—ras突变定量值,评价其对胰腺癌辅助诊断的价值。方法收集53例胰腺占位病变的超声内镜细针穿刺物,采用肽核酸(PNA)钳制实时定量PER的方法检测K—ras基因野生及突变拷贝数,根据临床综合诊断,与细胞学比较,评价其诊断价值。结果53例患者最后确诊为胰腺癌37例,非恶性胰腺占位16例,胰腺癌组K—ras基因的突变率为83.8%,非恶性胰腺占位组突变率为18.8%,两组之间比较差异有统计学意义(P〈0.05)。细胞学和K—ran定量检测诊断的灵敏度分别为59.5%和83.8%,将两者联合后诊断胰腺癌的灵敏度可提高至89.2%。结论胰腺组织超声内镜细针穿刺物中K—ras定量检查对胰腺癌有临床辅助诊断价值。 Objective To investigate the diagnostic value of a quantitative detection of K-ras muta- tion in samples from endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) of pancreatic cancer. Methods Samples taken by EUS-FNA from 53 pancreatic occupying lesions were collected, and the copies of wild-type and mutated K-ras gene was measured by PNA-elamping real-time quantitative PCR. The resuhs were analyzed with refer to cytological findings to evaluate its clinical values. Results According to cytological finding, a total of 37 cases were diagnosed as pancreatic cancer, and 16 were non-malignant lesions. Kras mutation was detected in 83.8% of cancer cases, and 18.8% of non-cancer cases, which was significantly different (P 〈0. 05). Sensitivities of cytology and K-ras examination were 59.5% and 83.8% , re- spectively, while that of combination of cytology and K-ras examination was 89. 2%. Conclusion Quantitative analysis of the mutant K-ras gene in samples taken by EUS-FNA is a useful tool for diagnosing the pancreatic carcinoma.
出处 《中华消化内镜杂志》 北大核心 2011年第3期126-130,共5页 Chinese Journal of Digestive Endoscopy
基金 国家科技支撑计划课题资助
关键词 胰腺癌 内窥镜超声检查 穿刺活检 基因 RAS Pancreatic cancer Endoscopic uhrasonography Puncture biopsy Genes, ras
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