摘要
目的探讨马钱子碱抗肝细胞癌的作用及其机制。方法体外培养人肝癌SMMC．7721细胞，加入不同浓度的马钱子碱（2．5～400μg∥m1），细胞培养72h，M1TT法测定细胞生长抑制率。Western blotting和荧光定量RT-PCR技术分别测定培养24、48、72h肝癌细胞PCNA、CyclinD1、FAS基因mRNA和蛋白表达。结果随着马钱子碱用量逐渐增加，对人肝癌细胞SMMC-7721生长抑制作用增强，马钱子碱用量为320仙g／ml时对肝癌细胞生长抑制率接近100％。马钱子碱作用肝癌细胞24h与作用48h相比，Fas蛋白和mRNA表达差异无统计学意义（分别F=2．547，1．582，均P〉0．05），作用72h时差异有统计学意义（分别F=1．036，1．137，均P〈0．05）；PCNA和CyclinD1的mRNA和蛋白表达各时间点差异无统计学意义（PCNA分别F=3．612，2．174，3．029；CyclinD1分别F=2．361，2．915，1．976，均P〉0．05）。结论马钱子碱抑制肝癌细胞生长，可能通过肝癌细胞FAS基因和蛋白表达增加．诱导肝癌细胞凋亡发挥抑制作用。而与PCNA和CvclinD1作用无关。
Objective To study the effect of brucine on the growth of a hepatocellular carcinoma cell line in vitro. Methods Brucine was added into a liver cancer cell line of SMMC-7721 in vitro, at drug concentration of brucine from 2. 5 μg/ml to 400μg/ml. The inhibition rate of cell growth was measured by MTF technique after the cells were cultured for 72 hours. The protein and mRNA expression of PCNA, cyelin D1 and FAS were respectively assayed with Western blotting and fluorescent quantitation RT-PCR techniques at 24, 48, 72 h. Results The inhibition rate of liver cancer cell was near 100% when the brucine concentration was at 320μg/ml. The protein and mRNA expression of FAS were of no significant difference at 24 h vs 48 h （ seperately F = 2. 547,1. 582, all P 〉 0. 05 ）, and significant difference existed at 24 h vs 72 h（seperately F = 1. 036, 1. 137, all P 〈0. 05）. The protein and mRNA expression of PCNA, Cyclin D1 were of no significant difference between various time period（ seperately PCNA F = 3. 612,2. 174, 3. 029 ;Cyclin D1 F = 2. 361,2. 915,1. 976, all P 〉 0. 05 ）. Conclusions Brucine inhibits the growth of liver cancer ceils, by inducing increased apoptosis of the ceils probably through FAS overexpression.
出处
《中华普通外科杂志》
CSCD
北大核心
2011年第3期219-221,共3页
Chinese Journal of General Surgery
基金
上海市教委基金资助项目（07CZ017）
国家自然科学基金资助项目（30873341）
关键词
癌
肝细胞
马钱子碱
药物筛选试验
抗肿瘤
Carcinoma, hepatocellular
Brucine
Drug screening assay, antitumor
