摘要
目的 分析结节病肉芽肿细胞（吞噬细胞、类上皮细胞、多核巨细胞）内空气细颗粒物（PM2.5）的元素组成及来源，探讨结节病与PM：，的相关性。方法收集50例结节病病变组织、10例非结节病成人尸检肺组织、18例PM25支气管灌注染毒大鼠肺组织（有肉芽肿病灶）石蜡包埋标本，采集大气中PM2.5，分别行HE染色、沃森一斯塔理（Warthin—Starry，WS）银染色，应用共聚焦显微镜拉曼光谱和透射电镜．能谱对上述部分病例标本肉芽肿细胞和尘细胞内的PM2.5，及大气PM2.5的成分进行分析，用X线荧光分析技术对所采集大气PM2.5的主要元素成分进行分析。结果结节病病变组织、非结节病成人尸检肺组织、PM2.5支气管灌注染毒大鼠肺组织及大气中PM2.5共4组标本中PM2.5的拉曼光谱和元素组成非常相似，均以碳质成分为主要特征。结论为结节病肉芽肿细胞中的PM2.5来源于大气PM2.5提供了进一步的依据，从而不能完全除外结节病患者可能是对PM2.5易感的个体的可能性。
Objective To explore the source of the fine particulate matter （PM2.5 ） in the sarcoidosis granulomatous cell and the relationship between the sarcoidosis and the PM2.5 in the atmosphere. Methods Paraffin-embedded tissues of 50 cases of human sarcoidosis biopsy samples, 10 cases of nonsareoidosis autopsy lung samples, 18 cases of lung tissues （with granulomatous lesions） of rats exposed to PM2.5 by bronchial infusion, and the free PM2.5 sample in the atmosphere were collected. The characteristics of tissues above mentioned were observed under the light microscopy, which stained by HE staining and Warthin-Starry silver staining. The characteristics of the PM2.5 in the four groups were analyzed using confocal Raman microscopy. The component of the PM2.5 in the sarcoidosis granuloma was analyzed using transmission electron microscope-energy dispersive X-ray detector （TEM-EDX） , and the component of the PM2.5 in the atmosphere was analyzed with X-ray fluorescence separately. Results The PM2.5 in the four groups have the similar Raman spectrum, they share the feature of carbonaceous composition, the element component of PM2.5 in the human sarcoidosis was the same as PM2.5 in the atmosphere. Conclusion The study provided the further evidence that the PM2.5 in the sarcoidosis lesion was from PM2.5 in the atmosphere, and it should be not excepted that sarcoidosis may be a sensitive individual reaction to the PM2.5 inhaled from the atmosphere.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2011年第3期177-181,共5页
Chinese Journal of Pathology
基金
北京市卫生系统高层次卫生技术人才培养项目（2009-3-31）
关键词
结节病
颗粒物
光谱分析
拉曼
Sarcoidosis
Particulate matter
Spectrum analysis, Raman
