摘要
目的探讨γ干扰素（ＩＦＮγ）基因修饰的肝细胞经脾移植抗血吸虫病肝纤维化作用。方法采用ＥＬＩＳＡ和免疫组化方法，动态分析日本血吸虫感染小鼠ＩＦＮγ和Ⅰ、Ⅲ型胶原水平及其相互关系，并将小鼠ＩＦＮγ重组腺病毒转染的肝细胞经脾移植到感染１６周小鼠，分析ＩＦＮγ基因治疗血吸虫病肝纤维化的作用。结果感染４周时，小鼠ＩＦＮγ水平明显升高，在感染１２周时达高峰；但在１６周以后ＩＦＮγ水平明显下降，而Ⅰ、Ⅲ型胶原的合成持续增高；ＩＦＮγ基因修饰的肝细胞经脾移植后能稳定有效地表达，显著降低Ⅰ、Ⅲ型胶原的合成、沉积，减轻肝纤维化程度。结论ＩＦＮγ可能与Ⅰ、Ⅲ型胶原的合成、沉积有关；ＩＦＮγ基因治疗能有效地发挥抗血吸虫病肝纤维化作用。
Objective To investigate the
efficacy of interferon (IFN) genemodified hepatocytes against hepatic fibrosis by intrasplenic
transplantion. Methods Levels of INF as well as type and collagen in the mice infected by
S.japonicum were dynamically analysed by ELISA and immunohistochemical assay.At the 16th
week,the infected mice were intrasplenic transplanted with murine hepatocytes transfected with
INF genecombinant adenovirus. Results At the 4th week of postinfection,murine INF leve was
increased markedly and reached to a peak at the 12th week,but decreased dramatically after the
16th week of postinfection,while the levels of type and collagen were increased continuously.
Meanwhile,the intrasplenic transplanted IFN genemodified hepatocytes steadily expressed IFN
and obviously reduced the synthesis and deposition of type and collagen as well as the
severity of hepatic fibrosis. Conclusion IFN may play an important role in decreasing the
production and deposition of type and collagen,while IFN gene transplantation can inhibit
hepatic fibrosis in Schistosomiasis japonica effectively.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1999年第3期166-168,共3页
Chinese Journal of Infectious Diseases
基金
国家自然科学基金
国家杰出青年科学基金
