摘要
目的观察不同亚治疗剂量吡喹酮对慢性血吸虫病患者血清血吸虫肠相关趋阴极抗原（ＧＡＣＡ）水平的影响。方法ＫａｔｏＫａｔｚ法粪检确定１０８例慢性血吸虫病患者，随机分成４组，分别给予口服５ｍｇ／ｋｇ、１０ｍｇ／ｋｇ、２０ｍｇ／ｋｇ剂量吡喹酮和安慰剂。采用单克隆抗体酶联免疫吸附试验（ＭｃＡｂＤｏｔＥＬＩＳＡ）分别检测服药前、后２、８、２４和４８小时患者血清ＧＡＣＡ水平。服药前、后检出率变化采用χ２检验。结果３个实验组服药后２小时检测血清ＧＡＣＡ水平，平均较服药前上升３个滴度，后渐下降；至服药后４８小时，血清ＧＡＣＡ水平仍高于服药前１个滴度。血清ＧＡＣＡ检测敏感性，可由服药前的８２．７％提高到服药后２小时的１００．０％（Ｐ＜０．０５）。对照组４８小时观察期内，血清ＧＡＣＡ水平波动在１个滴度范围内，检测敏感性无明显变化（Ｐ＞０．０５）。结论口服不同亚治疗剂量吡喹酮均能有效升高慢性血吸虫病患者血清血吸虫ＧＡＣＡ水平，并显著提高血清ＧＡＣＡ检测敏感性。
Objective To observe the effect of
different subclinical doses of praziquantel on the serum gutassociated cathodic antigen (GACA)
levels in the patients with chronic schistosomiasis. Methods One hundred and eight cases of
patients with chronic schistosomiasis diagnosed by KatoKatz method were divided into 4
groups,which were given praziquantel at the dosage of 5mg/kg,10mg/kg,20mg/kg and placebo
respectively.The serum samples were collected and detected by McAbDotELISA before
treatment and at 2h,8h,24h and 48h after treatment.Statistic analysis was 2 test. Results The
serum GACA levels of all three experimental groups at 2h after treatment were higher than that
before treatment by 3 titers.However,the titer was decreased gradually after 2h of the thereapy.
Till 48h after treatment the serum GACA levels were still 1 titer higher than that before treatment.
The positive detection rate of serum GACA was raised from 82.7% before treatment to 100.0% at
2h after treatment (P<0.05).Comparatively,there were no obvious changes on the serum GACA
levels and the positive detection rate in the control group (P>0.05) during the same period of
observation. Conclusion Different subclinical doses of praziquantel can increase the serum
GACA levels as well as the sensitivity of GACA detection effectively in the patients with chronic
schistosomiasis significantly.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1999年第3期169-171,共3页
Chinese Journal of Infectious Diseases
基金
世界银行贷款中国血吸虫病控制项目科研基金
关键词
血吸虫病
抗原
吡喹酮
免疫诊断
药物疗法
Chronic schistosomiasisGutassociated cathodic
antigenPraziquantelImmunodiagnosis HK
