摘要
目的：观察离体大鼠心脏缺血期补充一氧化氮（ＮＯ）供体对缺血再灌注损伤的影响。 方法：将２６只离体大鼠心脏缺血３０分，再灌注６０分。分为两组，用药组（１５只）及对照组（１１ 只）。用药组于缺血期给予４．４×１０－ ３ ｍｍ ｏｌ／Ｌ硝酸甘油（ｎｉｔｒｏｇｌｙｃｅｒｉｎ，ＮＴＧ，一种ＮＯ供体），碳酸氢盐缓冲液灌注。对照组仅给予碳酸氢盐缓冲液灌注。全部心脏均测定ＮＯ释放量、肌酸激酶漏出量及（或）心脏功能。 结果：用药组大鼠心脏，使用ＮＴＧ表现为两种效应。其中部分大鼠心脏（非心室颤动组，ｎ＝ ７），ＮＴＧ增加肌酸激酶漏出量，减弱再灌注期心脏功能的恢复，伴随缺血期ＮＯ释放量的增加。对另一部分大鼠心脏（心室颤动组，ｎ＝ ８），ＮＴＧ减少肌酸激酶的释放，但心脏于再灌注期持续心室颤动，缺血期ＮＯ释放量无明显增加。 结论：缺血期给予同一剂量ＮＴＧ对心肌缺血再灌注损伤产生双重效应，既可增加心肌损伤，又可减轻心肌损伤。
Objective:To examine whether the effect of supplementation of nitric oxide (NO) during ischemia is protective or damaging to the process of myocardial ischemia/reperfusion in the isolated rat hearts. Methods:Isolated perfused rat hearts( n =26) were subjected to 30 minutes of low flow global ischemia,followed by 60 minutes of reperfusion.In treated hearts( n =15),one dosage(4 4×10 -3 mmol/L) of nitroglycerin (NTG,as a NO donor) was infused during ischemia.Control hearts( n =11) were only infused with Krebs Henseleit bicarbonate buffer (KHBB) fluid.NO release,creatine kinase (CK) leakage and cardiac function (the rate pressure product) were measured in the hearts. Results:Two kinds of effects of NTG were found in the treated hearts.In some hearts( n=7 ),the CK leakage was increased and the postischemic recovery of cardiac function was reduced.The effects were accompanied by an increase of NO release during ischemia.In the other hearts ( n =8) ,the CK leakage was decreased,but the hearts exhibited continuous ventricular fibrillation after reperfusion without an apparent increase of NO release during ischemia. Conclusion:The results suggest that a same dose NTG(NO donor) infused during ischemia plays a dual role in myocardial ischemia and/or reperfusion injury.
出处
《中国循环杂志》
CSCD
北大核心
1999年第4期242-244,共3页
Chinese Circulation Journal
