摘要
目的：建立一种检测血清中硫酸阿米卡星的微生物检测方法,并用于临床监测。方法：以枯草芽孢杆菌作为检定菌,利用抗生素在一定的剂量范围内,抗生素的对数剂量与其所致的抑菌圈直径大小呈直线关系。结果：线性范围1-32μg/ml,线性回归方程：logC=0.1262D-1.8312,r=0.9989。检测方法稳定可靠。结论：此法操作简便,灵敏度高,可用于临床监测硫酸阿米卡星血药浓度。
Objective： To set up a method of detection for serum Amikacin sulfate by microorganism and to applicate it in clinic.Methods： Bacillus subtilis were used as the detected bacterium to determin the Amikacin dosage that the logarithm of Amikacin sulfate dosage and bacteriostasis diameter was linear relationship.Results： The Amikacin sulfate dosage was 1~32μg/ml and the equation of linear regression was logC=0.1262D-1.8312,r=0.9989.Conclusion： The method is convenient and sensibility.It can be used to detect Amikacin sulfate concentration in clinic.
出处
《华夏医学》
CAS
2010年第6期719-721,共3页
Acta Medicinae Sinica
基金
柳州市科学研究与技术开发计划项目(20040239)
关键词
硫酸阿米卡星
微生物法
浓度
Amikacin sulfate
bacterium method
concetration
