摘要
[Objective] The aim was to explore the molecular mechanisms of the A223C polymorphism at 3＇UTR of oxidized low density lipoprotein receptor 1（OLR1）which was closely related to milk fat percentage in cow.[Method]The putative microRNA targets located in the 3＇UTR of OLR1 were predicted with the software miRanda（1.0）which could carry the microRNA database of cow.[Result]Total 16 microRNA targets were found.The A223C SNP was located in complementary region of bta-miR-370 target sequence.[Conclusion]OLR1 3＇UTR A→C mutation had led to the disappearing of bta-miR-370 target,which had provided basis for the further research on the molecular mechanisms of the A223C polymorphism at 3＇UTR of OLR1 gene which was closely related to milk fat percentage in cow.
[目的]探索氧化型低密度脂蛋白受体OLR13′UTR A223C多态与奶牛乳脂率高低紧密关联的分子机制。[方法]用miRanda(1.0)软件装载牛microRNA数据库,预测OLR13′UTR潜在的microRNA。[结果]共预测16个microRNA靶标,OLR13′UTRA223C突变定位于bta-miR-370靶标种子序列的互补区。[结论]由于OLR13′UTRA→C突变导致bta-miR-370靶标的消失,为OLR13′UTR A223C多态与奶牛乳脂率高低紧密关联的分子机制深入研究提供依据。
基金
Supported by Key Project in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period(No.2008BADB2B04-8-4)
Natural Science Foundation of Hebei Province(No.C2008001308 and C2007000743)~~
