期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

钙调蛋白在PC12细胞分化和迁移中的作用 被引量：5

Effect of Calmodulin on the Differrentiation and Migration of PC12 Cells
下载PDF
导出
摘要 PC12细胞经神经生长因子(NGF)作用后,利用免疫荧光染色、单个细胞迁移率检测等方法,研究了钙调蛋白在PC12细胞中的分布以及钙调蛋白对PC12细胞分化和迁移的影响。免疫荧光染色结果表明,钙调蛋白在PC12细胞突起的顶端处呈密集分布。加入钙调蛋白抑制剂W7的细胞仅有少量长出突起。单个细胞迁移率检测表明钙调蛋白可能促进PC12细胞迁移。提示钙调蛋白可能在PC12细胞的分化和迁移过程中发挥作用。 To investigate the roles of calmodulin during neuronal differentiation and migration,we checked PC12 cells by immunofluorescence staining and single cell tracking assay after NGF treatment.We found that calmodulin showed a dense distribution pattern in top of PC12 cells.Only a small percentage of the cells grown in W7 treatment cells.A single cell tracking experiment showed that calmodulin in PC12 cells could increase cell motility.The data suggested that calmodulin may play an important role in differentiation and migration of PC12 cells.
作者 袁俊 李朝军
机构地区 南京晓庄学院生物化工与环境科学学院 南京大学医学院
出处 《华北农学报》 CSCD 北大核心 2011年第1期22-25,共4页 Acta Agriculturae Boreali-Sinica
关键词 钙调蛋白 PC12细胞 W7 分化 迁移 Calmodulin PC12 cell W7 Differentiation Migration
分类号 Q26 [生物学—细胞生物学]
  • 相关文献

参考文献11

  • 1Rhoads A R,Friedberg F. Seq'uence motifs for CaM recog- nition[J].FASEBJ,1997,11:331 - 340.
  • 2PowersJ F, Shahsavari M, Tsokas P, et al. Nerve growth factor receptor signaling in proliferation of normal adult rat chromaffin cells [ J ]. Cell Tissue Res, 1999,295 : 21 - 32.
  • 3徐娟,刘洪臣,徐璐璐.人牙周膜细胞条件培养基促PC12细胞分化的作用研究[J].南方医科大学学报,2010,30(3):549-551. 被引量：2
  • 4Yuan Jun,Shi Guo-Xin. Calmodulin bound to stress fibers but not microtubules involves regulation of cell morpholo- gy and motility[ J]. The International Journal of Biochem- istry & Cell Biology,2008,40:284 - 293.
  • 5Kaberi P D, Theresa M F, William R M. Assessment ofPC12 cell differentiation and neurite growth :a comparison of morphological and neurochemical measures [ J ]. Neuro- toxicol Teratol, 2004,26:397 - 401.
  • 6Butler T L, Kassed C A, Pennypacker K R. Signal trans- duction and neuro survival in experimental models of brain injury[J]. Brain Res Bu11,2003,59:339 - 351.
  • 7Yu Y Y, Chen Y, Dai G,et al. The association of CaM with central spindle regulates the initiation of cytokinesis in HeLa ceils [ J ]. International Journal of Biochemitry and Cell Biology ,2004,36( 8 ) : 1562 - 1572.
  • 8阎晓玲,苏心,张文治,王新平,秦进喜.rhEGF和rhbFGF诱导胎鼠脑组织神经干细胞向神经元样细胞分化的研究[J].中国现代神经疾病杂志,2008,8(3):246-251. 被引量：3
  • 9Li Y,Lin J L, Reiter R S,et al. Caldesmon mutant de- fective in Ca2-calmodulin binding interferes with as- sembly of stress fibers and affects cell morphology, growth and motility [ J]. J Cell Sci, 2004, 117 : 3593 - 3604.
  • 10Pfleiderer P J,Lu K K, Crow M T,et al. Modulation of vascular smooth muscle cell migration by calcium/calm- odulin-dependent protein kinase II-delta 2 [ J ]. Am J Physiol Cell Physio1,2004,286 : 1238 - 1245.

二级参考文献18

  • 1徐娟,刘洪臣,袁维秀,周红.NGF mRNA在人牙周膜成纤维细胞的表达[J].临床口腔医学杂志,2005,21(12):727-729. 被引量：3
  • 2Guo I, Ieyer SL, Kaur H, et al. Mutational studies of conserved residues in the dimmer interface of nerve growth factor[J]. Protein Sci, 1996, 5(3): 447-55.
  • 3Yan GZ, Ziff EB. NGF regulates the PC12 cell cycle machinery through specific inhibition of the Cdk kinases and induction of cyclin D1 [J].J Neurosci,1995, 15(9): 6200-12.
  • 4Ruit KG, Osborne PA, Schmidt RE, et al. Nerve growth factor regulates sympathetic ganglion cell morphology and survival in the adult mouse[J]. J Neurosci, 1990, 10(7): 2412-9.
  • 5Lewin GR, Barde YA. Physiology of the neurotrophins[J].Ann Rev Neurosci, 1996, 19: 289-317.
  • 6Sato J, Oerl ER. Adrenergic excitation of cutaneous pain receptors induced by peripheral nerve injury[J]. Science, 1991, 251 (5001): 1608-610.
  • 7Santos PM, Winterowd JG, Allen GG, et al. Nerve growth factor: increased angiogenesis without improved nerve regeneration [J]. Otolaryngol Head Neck Surg, 1991, 105(1): 12-25.
  • 8Maeda T, Ochi K, Nakakura-Ohshima K, et al. The Ruffini ending as the primary mechanoreeeptor in the periodontal ligament: its morphology, cytochemical features, regeneration and development [J]. Crit Rev Oral Biol Med, 1999, 10(3): 307-27.
  • 9Asaumi K, Nakanishi T, Asahara H, et al. Expression ofneurotrophins and their receptors (TRK) during fracture healing [J]. Bone, 2000, 26(6): 625-33.
  • 10[3]Buttke TM,McCuhrey JA,Owen TC.Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell lines.J Immunol Methods,1993,157(1/2):233-240.

共引文献3

同被引文献65

引证文献5

二级引证文献9

华北农学报
2011年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部