摘要
目的:探讨糖肾方(黄芪、生地、三七、鬼箭羽、大黄、山茱萸)对糖尿病肾病患者嘌呤及嘧啶代谢的影响。方法:对临床上确诊的糖尿病肾病患者,使用糖肾方进行治疗,在治疗0、3和6个月分别取血浆样本,使用HPLC-UV-MS/MS对血浆中肌酐、肌氨酸和19种嘌呤嘧啶循环相关代谢物进行定量分析,观察治疗过程中这些代谢物的变化。结果:在治疗过程中,肌酐、尿酸、黄嘌呤、肌苷、胞嘧啶和胸腺嘧啶核苷的含量均有显著性降低。结论:糖肾方对糖尿病肾病患者的嘌呤和嘧啶代谢有重要影响,其治疗糖尿病肾病的机理可能来自它对于体内氧化应激以及肾脏血流动力学的调控。
AIM：To observe the effect of Tangshen Formula（Radix Astragali,Radix Rehmanniae Recens,Ra-dix et Rhizome Notoginseng,Ramulus Euonymi,Radix et Rhizoma Rhei,Fructus Corni） on the purine and pyrimi-dine metabolism of patients with diabetic nephropathy.METHODS：Plasma samples from the patients with diabet-ic nephropathy were taken at the treatment of current months,third months and sixth months.Then creatinine,sar-cosine and nineteen metabolites involved in purine and pyrimidine metabolism were quantified using HPLC-UV-MS/ MS.With the quantitive analysis,changes of metabolites in the concentration were observed during the treatment.RESULTS：During the treatment,the levels of creatinine,uric acid,xanthine,inosine,cytosine and thymidine decreased significantly.CONCLUSION：Tangshen Formula has effects on the purine and pyrimidine metabolism in patients with diabetic nephropathy.The important mechanism of action may stem from the regulation and control over oxidative stress and hemodynamics in kidney.
出处
《中成药》
CAS
CSCD
北大核心
2011年第1期13-17,共5页
Chinese Traditional Patent Medicine
基金
科技部973计划专项课题(2005CB523503)资助
关键词
糖尿病肾病
糖肾方
嘌呤代谢
嘧啶代谢
定量分析
diabetic nephropathy； Tangshen Formula； purine metabolism； pyrimidine metabolism； quantitive analysis；
