摘要
目的了解血培养中病原菌的分布及药敏率。方法用Bact/Alert3D全自动血培养仪进行培养,VITEK-2 Compact微生物仪鉴定,纸片扩散法(K-B法)做药敏试验,采用WHONET5.4软件进行分析。结果临床送检的1578份血培养标本中,分离出病原菌257株,阳性率为16.3%,其中革兰阳性菌占41.6%,革兰阴性菌占40.1%,真菌占18.3%;检出率最高的病原菌分别为凝固酶阴性葡萄球菌(24.5%)、大肠埃希菌(24.1%)、白色假丝酵母菌(10.9%);检出菌数量位居前3位的病区分别为ICU(40.5%)、血液科(8.6%)、风湿免疫科(7.8%);革兰阳性球菌对替考拉宁、万古霉素、利奈唑胺的敏感率为100.0%;肠杆菌科对亚胺培南、美罗培南的敏感率为100.0%,对哌拉西林/他唑巴坦敏感率>80.0%;白色假丝酵母菌对氟康唑和伊曲康唑敏感率分别为82.1%、71.4%。结论血培养分离的病原菌种类分布广,以条件致病菌为主,对常用抗菌药物耐药明显,对血培养分离株进行早期检测和耐药性分析非常重要,可以为临床诊断和选择抗菌药物提供可靠依据,防止抗菌药物滥用及耐药菌株的产生。
OBJECTIVE To investigate the distribute of pathogenic bacteria in blood culture,and observe the drug resistance of common pathogenic bacteria in our hospital.METHODS Blood samples were cultivated with Bact/Alert3D automated hemanalysis system,pathogenic bacteria were identified with VITEK 2 compact,drug sensitivity test was carried out by using K-B method and the test results were analyzed with WHONET 5.4 software.RESULTS Among the 1578 clinical blood samples,257 strains of pathogenic bacteria were isolated,the positive rate was 16.3%,Gram-positive bacteria were the most common pathogens（41.6%）,then Gram-negative bacteria（40.1%） and fungi（18.3%）.The coagulase negative staphylococcus were the most common in all bacteria（24.5%）,then E.coli（24.1%） and Candida albicans（10.9%）.The pathogenic bacteria were isolated from ICU sickroom（40.5%） then Blood internal medicine sickroom（8.6%） and Rheumatism immunity sickroom（7.8%）.The Gram-positive bacteria were sensitve to Teicoplanin,Vanconmycin and linezolid.The Gram-negative bacteria were sensitive to imipenem,meropenem and piperacillin/tazobactam;the sensitivity of C.albicans to Fluconazole,the sensitivity of Candida glabrate and Candida tropicalis to Itraconazole.CONCLUSION The microorganisms isolated from blood culture are widely distributed and the drug resistance to common antibiotics is high,the early detection of the isolated pathogens and the drug resistance analysis are very important in guiding the clinical administration.It suggests that antibiotic should be used rationally so as to reduce the drug resistance of bacteria.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第7期1472-1474,共3页
Chinese Journal of Nosocomiology
关键词
血培养
病原菌
耐药性
Blood culture
Pathogenic bacteria
Drug resistance
