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禽肠炎沙门菌外膜蛋白OMPX基因的克隆及其在乳酸乳球菌中的表达 被引量:4

Cloning and expression of Salmonella enteritidis OMPX gene in Lactococcus lactis
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摘要 以禽肠炎沙门菌基因组DNA为模板,采用PCR技术扩增得到外膜蛋白OMPX基因片段,并将其克隆到乳酸乳球菌表达载体pMG36e中,构建重组质粒pMG36e-OMPX。将重组质粒电转入乳酸乳球菌MG1614,得到重组基因工程乳酸乳球菌,在GM17培养基中培养12h后,经SDS-PAGE分析显示表达的蛋白约为16 000,与预期相符,经Western-blot检测表明,表达的蛋白具有良好的反应特异性。 To clone and express the gene encoded the outer membrane protein X of Salmonella enteritidis.The PCR product was cloned into pMG36e vector,recombinant expression plasmid pMG36e-OMPX was constructed.Then the recombinants were transferred into the host strain MG1614,after cultured 12 hours in GM17 medium,SDS-PAGE showed a 16 000 protein was expressed and Western-blot confirmed the antigenicity against positive sera.The recombinant protein could be useful resource for development of genetic engineering Lactobacillus.
出处 《中国兽医学报》 CAS CSCD 北大核心 2011年第4期489-492,共4页 Chinese Journal of Veterinary Science
基金 国家现代农业产业技术体系建设专项资金资助 山东省科技支撑计划资助项目(2007GG20009001) 国家"十一五"科技支撑计划重大项目(2006BAK02A21)
关键词 沙门菌 外膜蛋白 乳酸乳球菌 表达 Salmonella enteritidis outer membrane protein X Lactococcus lactis expression
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