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牛整合素β5亚基基因的构建与原核表达

Cloning and Expression of Integrin Beta 5 Subunit Gene of Bos Tauros
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摘要 [目的]构建牛整合素β5亚基(ITGB5)基因,并进行原核表达。[方法]扩增目的基因,将其重组到pET-32a(+)质粒中,将经鉴定为阳性的重组克隆,转化大肠杆菌感受态细胞表达蛋白后纯化。[结果]从牛甲状腺、扁桃体扩增到目的基因ITGB5;ITGB5基因表达产物的SDS-PAGE结果显示,在88 KD处出现表达产物及纯化后的蛋白;W estern-blot分析表明,抗血清可与原核表达的蛋白特异性结合。[结论]表达、并纯化了ITGB5蛋白。 [Objective] To clone and express integrin beta 5 subunit（ITGB5）gene of bos tauros.[Method]Objective gene was obtained,and cloned into pET-32a（＋） vector.The recombinant plasmid was transformed into E.coli BL21 to express,then the expected protein was purified.[Result] ITGB5 gene was obtained from thyroid and tonsils of bos tauros.An about 88 KD expected protein was determined by SDS-PAGE.And the result of Western-blot showed that the expected protein could be combined by specific antibody.[Conclusion]The ITGB5 protein was expressed and purified.
作者 陈旭 王洪梅 武建明 刘晓 王立群 何洪彬
机构地区 东北农业大学 山东省农业科学院奶牛研究中心
出处 《安徽农业科学》 CAS 北大核心 2011年第7期4009-4011,共3页 Journal of Anhui Agricultural Sciences
基金 国家转基因重大专项(2009ZX08007-006B) 国家自然科学基金(31072160) 山东省农业重大应用技术创新课题 山东省科技攻关课题(2009GG20002032) 山东省自然基金(Y2008D20) 济南市高校院所自主创新计划(201004027)
关键词 牛 整合素β5亚基 基因 表达 Bos Tauros Integrin beta 5 Subunit Gene Expression
分类号 S188 [农业科学—农业基础科学]
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参考文献10

  • 1COX D, BRENNAN M,MORAN N. lntegrins as therapeutic targets: lessons and opporlunities [ J ]. Nat Rev Drag Discoy,' ,2010,9(10) :804 - 820.
  • 2WANG H, LIM D, RUDD C E. Inununopathologies linked to integrin sig- nalling[ J]. Semin hnmunopathol ,.2010,32(2) :173 - 182.
  • 3RUIZ-SAENZ J,GOEZ Y, TABARES W, et al. Cellular receptors for foot and mouth disease virus[J]. Intervirology,2009,52(4):201 -212.
  • 4BURMAN A,CLARK S,ABRESCIA N G,et al. Specificity of the VP1 GH loop of foot-and-mouth disease virus for alphav integrins[J]. J Viro|,2006, 80(19) :9798 -9810.
  • 5BERINSTEIN A ,ROIVAINEN M ,HOVI T,et al. Antibodies to the vitrone- ctin receptor (integrin ct V β 3 ) inhibit binding and infection of foot-and- mouth diseatse vin*s to cuhured cells[J]. Journal of Virology,1995,69(4) : 2664 - 2666.
  • 6O'DONNELL V, PACHECO J M, GREGG D, et 'al. Analysis of foot-and- mouth disease virus integrin receptor expression in tissues from naive mad infected cattle [ J ]. J Comp Patho1,2009,141 (2/3) :98 - 112.
  • 7JOHNS H L,BERRYMAN S,MONAGHAN P,et al. A dominant-negative nmtmlt of rab5 inhibits infetion of cel|s by foot-and-mouth disease vires: implications for vires ent .ry[ J]. J Viro1,2009,83(12) :6247 -6256.
  • 8GLTIERRF1-RIYAS M,PUIJDO M R,BARANOWSKI E,et al. Tolerance to mutations in the foot-and-mouth disease virus integrin-binding RGD re- gion is different in cultured cells and in vivo and depends on the capsid sequence context[J]. J Get~ Viro1,2008,89(10) :2531 -2539.
  • 9JACKSON T, CLARK S, BERRYMAN S, et al. lntegrin alphavbeta8 func- tions ms a receptor fbr foot-and-mouth disease virus : role of the beta-chain cyt(xlomain in integrin-mediated itffection [ J ]. J Viro1,2004,78 (9) :4533 - 4540.
  • 10BROWN J K,MCALEESE S M,THORNrON E M,et al. Integin-alphav- beta6,a putative receptor for foot-and-mouth disease virus,is constitutive- ly expressed in ruminant airways [ J ]. J Histochem Cytochem, 2006,54 (7) :807 -816.
安徽农业科学
2011年 第7期

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