摘要
从分子水平上探讨海洋新药甘糖酯的抗栓作用机理。实验以大鼠为材料，采用定量ＲＴＰＣＲ方法定量检测口服甘糖酯后大鼠尿激酶型纤溶酶原激活物（ｕＰＡ）基因ｍ ＲＮＡ水平变化。研究表明大鼠口服甘糖酯后ｕＰＡ基因ｍ ＲＮＡ 水平高于对照组，差异显著。结论认为甘糖酯可提高体内ｕＰＡ基因的ｍ ＲＮＡ 水平，从而提高ｕＰＡ蛋白的活性，激活机体的纤溶系统，达到抗栓作用。
Purpose: To understand the pharmacological mechanism of Propylene glyco mannate sulfacte (PGMS), which is known as an anti clot marine drug. Method: Rats were used as material, and quantitative RT PCR was employed to assay the rat mRNA level of urokinase Plasminogen Activator (uPA) gene. Result: The mRNA levels of the experimental group were remarkably higher than those of the control. Conclusion: PGMS can rise the mRNA level of uPA gene, which in turn rises the uPA activity in the blood. That leads to the activation of fibrinolysis process.
基金
国家"九五"攻关项目
关键词
甘糖酯
大鼠
尿激酶型
纤溶酶原激活物
Propylene glyco mannate sulfate (PGMS)
Rat
urokinase Plasminogen Activator (uPA)
RT PCR
