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菟丝子含药血清促进骨髓间充质干细胞增殖的效应及机制 被引量:30

Effect and mechanism of dodder medicated serum on MSCs proliferation in vitro
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摘要 目的:探讨菟丝子含药血清体外对骨髓间充质干细胞(MSCs)增殖的影响及机制研究。方法:分离、培养大鼠MSCs,菟丝子含药血清诱导培养MSCs 72h后,MTT法检测大鼠MSCs增殖情况;RT-PCR检测增殖过程中MSCs不同细胞因子mRNA的表达;Real-time PCR检测骨形态蛋白-2(BMP-2)mRNA表达;Western-blot检测BMP-2蛋白表达。结果:10%菟丝子含药血清具有显著促进MSCs增殖的作用(P<0.05),并显著促进BMP-2 mRNA的表达(P<0.05)。结论:10%菟丝子含药血清促进MSCs增殖的作用,可能与其促进BMP-2 mRNA表达有关。 Objective:To investigate the effect and mechanism of dodder medicated serum on bone marrow mesenchymal stem cells(MSCs) proliferation in vitro.Methods:The dodder medicated serum-induced proliferation effect on MSCs was mesured by MTT;different cytokine mRNA expression was detected by RT-PCR,BMP-2 mRNA expression was analyzed by Real-time PCR;BMP-2 protein expression was detected by western blot.Results:Compared with the blank serum group,10%dodder medicated serum can significantly promote the proliferation of MSCs,and markedlly improved BMP-2 mRNA expression.Conclusion:10% dodder medicated serum can promote the proliferation of MSCs,and the mechanism involves the stimulation of BMP-2 mRNA expression.
机构地区 广州中医药大学
出处 《中华中医药杂志》 CAS CSCD 北大核心 2011年第4期818-822,共5页 China Journal of Traditional Chinese Medicine and Pharmacy
基金 国家自然基金课题(No.30500645)~~
关键词 菟丝子 含药血清 骨髓间充质干细胞 增殖 骨形态蛋白 Dodder Medicated serum MSCs Proliferation BMP-2
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