摘要
通过PCR的方法,扩增了松江鲈Myostatin基因的内含子1和内含子2片段,测序后发现长度分别为325bp和835bp,具有典型的GT-AG序列特征,在内含子2中存在一个(AC)14的微卫星序列.通过线粒体控制区部分片段测序,对来自长江、丹东和葫芦岛三个地理群体的松江鲈线粒体D-loop序列分析,未发现它们之间存在特征性的序列差异,可能是三个地理群体松江鲈的分隔时间短.
The intron 1 and intron 2 of myostatin gene in Trachidermus fasciatus were amplified and sequenced.The two introns are 325bp and 835 bp long respectively and contained the typical GT-AG ends of erkaryotic genes.Meanwhile the intron 2 contained a（AC）14 microsatellite sequence.The mitochondrial control regions of three geographic population of Trachidermus fasciatus from Yangtse River,Dandong,and Huludao were amplified and sequenced.There is no characteristic variation among these individuals,which showed that these three geographic populations of Trachidermus fasciatus were probably insulated for a short time.
出处
《常熟理工学院学报》
2011年第2期64-68,共5页
Journal of Changshu Institute of Technology
基金
苏州市2010年度科技基础设施建设项目(SZSD201001)
常熟理工学院2010大学生创新实践计划项目
江苏省高校自然科学研究计划(09KJD240001)
关键词
松江鲈
肌肉生长抑制素基因
线粒体控制区
Trachidermus fasciatus
Myostatin gene
mitochondrial control region
