摘要
目的探讨脂多糖致新生鼠脑白质损伤(PVL)后Bcl-2、P53蛋白的变化。方法新生2日龄Wistar大鼠100只,随机分为2组:对照组和PVL组,每组50只。对照组给予生理盐水5mg/kg腹腔注射,PVL组给予脂多糖5mg/kg腹腔注射。免疫组化染色观察术后6h、12h、24h、48hBcl-2及P53蛋白的变化。结果 PVL组Bcl-2蛋白表达6h升高,12h明显升高,48h达高峰,与对照组同一时间点比较均有非常显著性差异(P<0.01);P53蛋白表达6h明显升高,12h达高峰,与对照组同一时间点比较均有非常显著性差异(P<0.01)。结论脂多糖致早期新生鼠脑白质损伤增加Bcl-2和P53的表达。
Objective To investigate the change of Bcl-2 and P53 expresssion after whiter matter damage in neonatal rats. Methods100 rats （2 days old） were randomly divided into two groups： control group and periventricular leucumalacia （PVL） group. Control group received normal saline 5 mg/kg intraperitoneal injection, PVL group received lipopolysaccharide （LPS） 5 mg/kg intraperitoneal injection. The expression of Bcl-2 and P53 was measured 6 h, 12 h, 24 h, 48 h after operation with immunohistochemical staining. ResultsThe expression of Bcl-2 increased 6 h after operation, and to the top 48 h after operation in the PVL group, which was significantly different compared with the control group （P0.01）. The expression of P53 increased at 6 h after operation, and to the top 12 h after operation in the PVL group, which was significantly different compared with the control group （P0.01）. ConclusionThe expression of Bcl-2 and P53 increased in the white matter injuried neonatal rats induced by LPS.
出处
《中国康复理论与实践》
CSCD
2011年第3期240-242,共3页
Chinese Journal of Rehabilitation Theory and Practice
