摘要
目的:筛选皱瘤海鞘体外抗单纯疱疹病毒2型的有效部位。方法:应用单纯疱疹病毒2型在Vero细胞上的增殖为模型,运用细胞病变法(CPE法)和四甲基偶氮唑盐比色法(MTT比色法),评价皱瘤海鞘不同部位体外抗单纯疱疹病毒2型的药效学活性。结果:水溶性部位显示出明显的抗单纯疱疹病毒2型作用,而氯仿部位和正丁醇部位作用不明显。结论:皱瘤海鞘提取物在体外具有抗单纯疱疹病毒2型作用,有效部位是水溶性成分。
Objective： To screen effective fractions for anti-Herpes simplex virus 2 from Styela plicata.Methods： The model that Herpes simplex virus 2 proliferate in vero cells was applied,Cytopathic effect assay and MTT assay was applied to evaluate the effect of defferent fraction from Styela plicata on Herpes simplex virus 2 in vitro.Results： Anti Herpes simplex virus 2 was demonstrated in water-soluble fraction,and there was no significant effcet in chloroform and n-butanol fractions.Conclusions ： Extractions from Styela plicata has anti-Herpes simplex virus 2 effect,and the effective fraction may be the water-soluble components.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2010年第6期61-63,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
广州市科技计划项目(2005J1-C0401)
