摘要
目的:研究细胞因子LIF、EGF、bFGF和肝素钠,以及氧分压、温度等多因素对昆明系小鼠胚胎干细胞(KM-ESC)原代集落形成和后期增殖的影响。方法:本研究选择LIF、EGF、bFGF、肝素钠、5%O2,20%O2和37℃、39℃作为研究条件。并检测不同情况下小鼠胚胎干细胞原代集落形成和后期增殖情况。结果:LIF对KM-ESC原代集落形成和后期增殖具有显著促进作用,极显著高于EGF、bFGF和肝素钠组(P<0.01)。温度对KM-ESC原代集落形成和增殖具有显著影响,39℃条件下,原代集落形成率、直径和后期增殖显著高于37℃(P<0.05);而氧分压对KM-ESC原代集落形成无显著作用(P>0.05),但是对原代集落直径和后期增殖有一定促进作用,20%O2组显著高于5%O2组(P<0.05)。结论:LIF、EGF、bFGF、肝素钠、39℃、20%O2对小鼠胚胎干细胞原代集落形成和后期增殖具有显著促进作用。
Objective：To investigate the effects of several factors such as cytokines（LIF,EGF,bFGF）,heparin sodium,Oxygen Stressand,temperature on colony formation and post growth of primary Kunming mouse embryonic stem cell（KM-ESC）.Method：LIF,EGF,bFGF,5% O2,heparin sodium,20% O2 Oxygen Stressand and 37℃,39℃ were used in this study.Colony formation and post growth of primary Kunming mouse embryonic stem cell（KM-ESC） in LIF,EGF,bFGF,heparin sodium,Oxygen Stressand,temperature were detected.Result：LIF has a significant promoting function on colony formation and post growth of primary KM-ESC,on which the temperature has a significant effect,higher than on EGF,bFGF and the group of heparin sodium.（P0.01） Under the condition of 39℃,the colony formation rate,diameter and post growth are higher than 37℃（P0.05）.Oxygen Stress has no effect on colony formation of KM-ESC（P0.05）,but has a promote effect on colony diameter and post growth.The group of 20% O2 is a little higher than the group of 5% O2（P0.05）.Conclusion：LIF,EGF,bFGF,5% O2,heparin sodium,20% O2 Oxygen Stressand and 39℃ had promoting function on colony formation and post growth of primary KM-ESC.
出处
《现代生物医学进展》
CAS
2011年第4期611-614,共4页
Progress in Modern Biomedicine
基金
国家"十一五"863计划项目(2008aa101005)
新疆维吾尔自治区高技术研究发展计划项目(200711104)
新疆畜牧科学院青年科研基金项目(2008QJ01
2010QJ006)资助
中国博士后科学基金项目(200801450)
关键词
胚胎干细胞
细胞因子
温度
氧分压
ESC
Cytokine
Temperature
Oxygen Partial Pressure
