摘要
目的:了解真菌感染的临床分布、真菌种类及耐药情况,为临床诊治提供依据。方法:采用沙保弱平板或CHROMa-gar平板进行菌株分离、API Candida、API20CAUX菌株鉴定,用Rosco纸片扩散法测定药敏。结果:690份标本,分离出700株真菌,其中假丝酵母菌属占53.5%,白假丝酵母菌占25.6%;痰标本占24.3%;住院患者中以ICU和肾内科分离率高且以白假丝酵母菌为主,分别占59.3%和45.2%;门诊患者以皮肤科分离率高,以近平滑假丝酵母菌为主,占23.4%;酵母样真菌对伊曲康唑、氟康唑、两性霉素B、制霉菌素、酮康唑的敏感率分别为89.4%、83.9%、98.2%、95.9%、96.0%。结论:假丝酵母菌是真菌培养中分离率最高的真菌,分离的主要真菌种类因科室和患者而异。真菌的分离、鉴定和药敏试验对临床使用抗真菌药物具有指导意义,特别是对氟康唑天然耐药菌株的鉴定,有利于抗真菌药物选择。
Objective：To analyze the antifungal susceptibility and fungi distribution in clinical.Methods： Sabouraud agar and CHROMagar were used to culture fungi.Identifications of fungi strains were detected by API Candida and API20C AUX.Antifungal susceptibility was determined by Rosco disc agar diffusion method.Results： Candida was 53.5%,of which C.albicans was 25.6% in total of 700 clinical isolates;C.albicans were mainly isolated from ICU and Dept.of nephrology with 59.3% and 45.2% respectively;C.parapsilosis was main isolated from Dept.of Dermatology.Antifungal suscepitibility rates of Yeast-like fungi to itraconazole,fluconazole,amphotericin B,nystatin,ketoconazole were 89.4%,83.9%,98.2%,95.9% and 96.0% respectively.Conclusion： Candida spp.is the main fungi isolated from clinical.Species of fungi distribution depends on disease category of patients and the part of the body infected.To identify fungi accurately is important to avoid use fluconale for fluconale-resistant strain.Antifungal susceptibility test is helpful to know trends of antifungal resistance.
出处
《四川生理科学杂志》
2011年第1期20-23,共4页
Sichuan Journal of Physiological Sciences
基金
深圳市科技计划项目(2000702108
200703031)
关键词
真菌
分布
抗真菌药
敏感性
Fungi； Distribution； Antifungal agents； Susceptibility；
