摘要
【目的】检测新疆芜菁多糖的组分结构与对血糖的作用。【方法】采用常规的水提法从芜菁中提取粗多糖,并用Sevag法对其进行脱蛋白,再通过回流、透析等步骤把粗多糖纯化获得芜菁精制多糖。用芜菁精制多糖进行小白鼠血糖实验。对芜菁精制多糖进行DEAE-52离子交换柱层析纯化,对第一个洗脱组分BRPS1再进行Sephadex G-200凝胶柱层析纯度鉴定。此外,用红外光谱法分析BRPS1,并且对离子交换柱层析的7种洗脱组分分别进行了GC-MS分析。【结果】当芜菁精制多糖剂量为400 mg/kg体重时,3 d后的受试小鼠血糖值下降差异显著(P<0.05);6和9 d后的血糖值下降差异极显著(分别是P<0.01和P<0.001);通过DEAE-52离子交换柱层析共分离出7种组分。对第一个洗脱组分BRPS1的Sephadex G-200凝胶柱层析的结果表明,BRPS1是单一的多糖组分;其红外图谱结果表明BRPS1具有多糖的一般结构。对7种洗脱组分的GC-MS分析表明芜菁精制多糖含有6种单糖。【结论】芜菁多糖具有显著的降血糖效果;作为芜菁多糖主要成分的BRPS1为单纯的多糖,具有多糖的一般结构。
【Objective】To investigate the composition and structure of polysaccharides from Brassica rapa L.of Xinjiang and its effects on blood sugar level.【Method】Crude polysaccharide was extracted from Brassica rapa L.by common water extraction method,and then deproteinized by Sevag method.The crude polysaccharide from Brassica rapa L.was further purified by refluxing and dialyzing,and then purified polysaccharide was obtained.Blood sugar level test was performed on white mice by using purified polysaccharide.DEAE-52 ion-exchange column chromatography was performed on purified polysaccharide and Sephadex G-200 gel column chromatography was performed on the first component BRPS1 for purity analysis.And then infrared analysis was performed on BRPS1;seven components obtained by ion-exchange column chromatography were analyzed by GC-MS for determining its monosaccharide composition.【Result】Under the dose of 400 mg/kg bw： after 3 days the blood sugar level decreased relatively obviously（P0.05）; after 6 days and 9 days the blood sugar level decreased quite obviously（P0.01 and P0.001 respectively）.Seven components were derived by DEAE-52 ion-exchange column chromatography.Result of Sephadex G-200 gel column chromatography showed that BRPS1 was pure polysaccharide.Infrared analysis indicated that BRPS1 had common composition of polysaccharides.Six kinds of monosaccharides were identified in Brassica rapa L. purified polysaccharides by GC-MS.【Conclusion】Polysaccharides from Brassica rapa L.of Xinjiang has obvious hypoglycemic effect;BRPS1,as the main component of Brassica rapa L.polysaccharides,is pure polysaccharide,and it has the common structure of polysaccharides.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2011年第3期471-479,共9页
Xinjiang Agricultural Sciences
基金
新疆大学植物生物技术重点实验室
关键词
芜菁
多糖
血糖测定
GC-MS
Brassica rapa L.
polysaccharide
blood sugar checking
GC-MS
