摘要
目的观察寒潮时易卒中型肾血管性高血压大鼠(RHRSP)血液中凝血和纤溶系统的活性改变。方法双肾双夹法制作RHRSP模型,分别于寒潮前后取血,酶联免疫吸附测定(ELISA)法检测P-选择素(Ps)、纤维蛋白肽A(FPA)和D-二聚体(DD)含量。结果造模8周后RHRSP血浆中Ps、FPA、DD含量逐渐增高,与假手术组相比差异有统计学意义(P<0.05)。且Ps、FPA、DD水平在寒潮3 h最高,6 h1、2 h逐渐下降,与RHRSP非寒潮组相比差异有统计学意义(P<0.05)。结论寒潮时血液中凝血系统和纤溶系统活性改变可能是RHRSP出现脑梗死和/或脑出血,甚至混合性中风的原因之一。
Objective To study the effect of artificial cold stream on coagulation and fibrinolysis in rats with stroke-prone renovascular hypertension.Methods The models with stroke-prone renovascular hypertension were established by two-kidney-two-clip.The content of P-selection,fibrinopeptides A,and D-dimer were measured by enzyme-linked immunosorbant assay before and after the cold stream.Results The contents of plasma P-selection,fibrinopeptides A and D-dimer were increased gradually at 8th week after operation,and they were different significantly compared with that in sham operation group（P〈0.05）.The levels of these factors were the highest at 3 h in artificial cold stream and decreased gradually at 6 h,12 h.They were different significantly compared with non-cold stream group（P〈0.05）.Conclusion Up-regulative activity of coagulation and fibrinolysis after artificial cold stream might play a role in the onset of cerebral infarction,cerebral hemorrhage,and mixed stroke.
出处
《中西医结合心脑血管病杂志》
2011年第4期459-462,共4页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
