摘要
用聚乙烯醇包埋、硫酸钠固定法对根霉（ Ｒｈｉｚｏｐｕｓｓｐ．Ｂｃ０－０９）菌体细胞进行了固定化，所得凝胶具有良好的机械性和稳定性． 对固定化细胞催化（ Ｒ，Ｓ）－环氧丙醇丁酸酯水解反应的性能进行了研究． 对ｐＨ值的影响考察结果表明， ３０ ℃下， 反应初速度在ｐＨ 值为５．５ 时最大； 酶的立体选择性在ｐＨ值为５．５～６．０ 时最高， 对映体比率（ Ｅ值）为５７． 对温度的影响考察结果表明，ｐＨ 值为５．５， 反应初速度在５５ ℃时最大； 酶的立体选择性随温度升高而下降． 固定化细胞具有较好的操作稳定性．
A new cell immobilization method was developed in which cells of Rhizopus sp. Bc0\|09 were immobilized by entrapment with polyvinyl alcohol\|sodium sulfate gel. The gel beads have high mechanical strength and operational stability. The properties of the immobilized cells in the optical resolution of glycidyl butyrate were investigated. The study on the effects of pH value showed that at 30 ℃, the initial rate of hydrolysis is the highest at pH value \{5.5;\} the enzyme stereoselectivity is higher at pH value 5.5～6.0, the enantiomeric ratio (E value) is 57. The effects of temperature showed that at pH value 5.5, the initial rate is the highest at 55 ℃ and the enzyme stereoselectivity decreased slightly with the rising of temperature.
出处
《分子催化》
EI
CAS
CSCD
北大核心
1999年第5期339-344,共6页
Journal of Molecular Catalysis(China)
基金
国家"九五"攻关课题
关键词
环氧丙醇丁酸酯
光学拆分
根霉
固定化细胞
R,S\|glycidyl butyrate
Optical resolution
Rhizopus
Immobilized cells
Polyvinyl alcohol
