摘要
在超临界CO2状态下,采用脂肪酶催化共轭亚油酸(conjugated linoleic acid,CLA)与甘油反应制备共轭亚油酸甘油酯,分别应用单因素和正交试验考察分子筛添加量、酶用量、反应压力、温度和时间对CLA酯化率的影响。结果表明,最佳工艺条件为分子筛用量6%、酶用量4%、反应温度60℃、反应时间20h、反应压力11MPa,此条件下CLA的酯化率可达到90.98%。这种CLA甘油酯的脂肪酸组成中,9c,11t-CLA和10t,12c-CLA的含量分别为37.79%和41.66%。
The lipase-catalyzed preparation of conjugated linoleic acid glyceride（CLA）from conjugated linoleic acid and glycerin under supercritical CO2 was studied.The effects of the quantities of molecular sieve and enzyme addition,pressure,temperature and time on the esterification rate of CLA were investigated.The results showed that the optimal parameters for preparing CLA were as follows：in the attendance of 6%type 3A molecular sieve and 4%Novozym 435 lipase,reaction at 60℃and 11 MPa for 20 h.Under these conditions,the esterification rate of CLA reached 90.98%.The contents of 9c,11t-CLA and 10t,12c-CLA was 37.79%and 41.66%,respectively in the CLA glyceride prepared.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第8期29-32,共4页
Food Science
基金
国家"863"计划项目(2010AA101503)
关键词
超临界CO2
共轭亚油酸
甘油酯
CLA酯化率
supercritical CO2
conjugated linoleic acid（CLA）
glyceride
esterification rate of CLA
