摘要
设计正交试验,以油橄榄叶片DNA(CTAB法提取)为模板,对影响油橄榄SSR-PCR反应的主要影响因素进行优化,建立适合油橄榄的PCR反应和DNA多态性检测体系。试验结果表明油橄榄最佳SSR-PCR反应体系为:20μl体系中含有DNA模板20ng、dNTP0.15mmol/L、引物0.15μmmol/L、Taq酶1.0U。同时对100对引物进行扩增试验,筛选出多态性好、条带清晰的引物24对,用于油橄榄遗传多样性分析。
Main factors affecting SSR-PCR system were optimized with the orthogonal design method, and the PCR reaction system and the detection system of DNA polymorpbisms for olive were established. The results showed the optimized SSR-PCR system was 20 ng DNA, 0.15 mmol/L dNTP, 0.15 μmmol/L SSR primer, 1.0 U Taq DNA polymerase in 20 μl content. 24 primer pairs were selected from100 primer pairs for the SSR analysis based on polymorphisms and clear banding patterns, which could be used for further olive genetic diversity research.
出处
《中国园艺文摘》
2011年第4期5-7,共3页
Chinese Horticulture Abstracts
基金
甘肃省自然基金项目(096RJZA024)
甘肃省科技厅资助
