摘要
采用响应面法(RSM)对影响酵母菌生长的培养条件进行优化。根据Box-benhnken中心组合试验设计原理,在单因素试验基础上采用4因素3水平的响应面分析法,建立了培养基吸光度值与各影响因子的回归方程,并得到以吸光度值为响应值的响应面图,进而得出培养酵母菌的的优化条件:培养基起始pH5.8、培养温度30℃、装液量95 mL/250 mL和接种量3.6%。验证试验结果表明,在此条件下培养基的吸光度值能达到1.676±0.03。
Optimization of the growth conditions of yeast by response surface method（RSM）.According to Box-benhnken central composite design,Basis on the single factor experiment,using 4 factors and 3 levels of response surface analysis method,a medium impact factor absorbance with the regression equation,and get to the absorbance value of response value of the response surface map,and then come to the optimization of yeast culture conditions： medium with initial pH5.8,culture temperature 30℃,liquid volume 95 mL/250 mL and the inoculation 3.6%.The test results showed that under these conditions the absorbance values of the medium can reach 1.676 ±0.03.
出处
《食品工业》
北大核心
2011年第4期36-39,共4页
The Food Industry
基金
北京市教委科技计划面上项目KM200800005001
关键词
响应面法
培养条件
优化
酵母菌
response surface method
culture conditions
optimization
yeast
