摘要
建立一种多重PCR法同时检测榛子磷酸葡糖异构酶、榛子致敏蛋白Cor a 1和Cor a 8三种蛋白的方法。通过琼脂糖凝胶电泳和凝胶成像系统对多重PCR的扩增结果进行分析。结果表明此方法具有良好的特异性,检出限可达10 ng/μL,且反应体系各组分未出现交叉干扰。经测序结果证实,三种扩增产物的序列与目的基因的序列高度一致,而且此方法的实样检测结果也比较理想,适合于检测食品中的榛子成分及榛子的主要致敏原。
A suitable multiple PCR method was developed for the detection of hazelnut cytosolic phosphoglucose isomerase,hazelnut major allergen Cor a 1 and Cor a 8.The results of multiple PCR were analyzed by agarose gel electrophoresis and Gel Doc XR system.With this method,the specific turned out to be very good and the detection limit could reach 10 ng/μL,besides this,the components of reaction system didn＇t show cross interference.Confirmed by the results of sequencing,the three PCR products showed high consistent with the target gene,and the result of real samples detection were also very good.This method is suitable for detection of the component of hazelnut and the main allergens of hazelnut.
出处
《食品工业》
北大核心
2011年第4期95-97,共3页
The Food Industry
关键词
榛子
磷酸葡糖异构酶
Cora1
Cora8
多重PCR
hazelnut
corylus heterophylla
cytosolic phosphoglucose isomerase
Cor a 1
Cor a 8
multiple PCR
