摘要
目的:建立一种准确简单易于普及的测定尿中砷的检测方法。方法:样品用混合酸在电热板上加热消解后,用硫脲抗坏血酸将五价砷预还原为三价砷,再用硼氢化钾作还原剂,以5%HNO3为介质,用AFS-230E原子荧光光度计直接测定。结果:在最佳实验条件下,砷的浓度在0.0μg/L^20.0μg/L之间线性关系良好,相关系数r=0.9999,方法的最低检出限为0.164μg/L,RSD为0.4%~1.2%,加标回收率为98.6%~103.5%。结论:该方法操作简单,精密度好,回收率高,是测定尿砷含量的可靠方法。
Objective：To establish an accurate and simple determination of universal detection of arsenic in urine.Methods： Mixed acid sample digestion in the electric panel heating with thiourea,ascorbic acid to pentavalent arsenic to trivalent arsenic pre-reduction,and then potassium borohydride as reducing agent,to 5% HNO3 as the medium,with the AFS-230E direct determination of atomic fluorescence spectrometer.Results： The optimal experimental conditions,good linearity was presented within the arsenic concentration range of 0.0 μg/L^20.0 μg/L;and the correlation coefficient was 0.9999,the lowest detection limit of the method was 0.164 μg/L,relative standard deviation（RSD） was 0.4%~1.2%,the recovery was 98.6%~103.5%.Conclusion： The method is simple,precise,which high recovery rate,which is to determine a reliable method of urinary arsenic.
出处
《中国卫生检验杂志》
CAS
2011年第4期856-858,共3页
Chinese Journal of Health Laboratory Technology
关键词
氢化物原子荧光法
湿法消化
硝酸
尿砷
Hydride generation atomic fluorescence spectrometry
Wet digestion
Nitric acid
Urine arsenic
