摘要
目的 观察黄芪多糖（APS）对2，4，6-三硝基苯磺酸（TNBS）诱导的实验性结肠炎大鼠肠道炎症及树突状细胞（DCs）的作用。方法44只雄性SD大鼠用简单随机抽样法分为4组（n=11）：空白对照组、TNBS模型组、APS治疗组、5-氨基水杨酸（5-ASA）治疗组。除空白对照组外，其余3组均给予TNBS灌肠造模。造模后第2天，APS治疗组和5-ASA治疗组分别给予APS（0．75g·kg^-1·d^-1）和5-ASA（100mg·kg^-1·d^-1）灌胃治疗10d。治疗结束后处死大鼠取材。对大鼠结肠炎症进行评估，包括疾病活动指数（DAI）评分、大体形态损伤评分、病理组织学评分和髓过氧化物酶（MPO）活性测定；流式细胞仪检测DCs表面标志MHCII和CD86的表达。结果APS治疗组较TNBS组大鼠的DAI评分（P=0．007）、大体形态损伤评分（P=0．017）、病理组织学评分（P=0．016）均显著降低，MPO活性亦下降，但差异无统计学意义（P=0．183）。TNBS模型组大鼠肠系膜淋巴结DCs表面标志MHCⅡ和CD86的表达率较空白对照组（P=0．005，P=0．008）、APS治疗组（P=0．023，P=0．018）及5-ASA治疗组（P=0．017，P=0．013）均显著升高。结论APS治疗可部分缓解TNBS诱导的大鼠实验性结肠炎，下调肠系膜淋巴结中活化的DCs。
Objective To investigate the effect of Astragalus polysaccharldes （APS） on ：2,4,6-trinitrobenzenesulfonic acid （TNBS）-induced colitis in rats and on dendritic cells （DCs） in mesenteric lymph nodes. Methods Forty-four male Sprague-Dawley rats were randomly divided into four groups （ n = 11 ） using simple random sampling： normal control group, TNBS group, APS group, and 5-aminosalicylic acid （5-ASA） group. Experimental colitis was induced in rats by TNBS enema in the last three groups. Rats in APS and 5-ASA groups were treated by gavage with APS （0. 75 g·kg^-1·d^-1） and 5-ASA （100 mg·kg^-1·d^-1） on the 10 consecutive days following TNBS administration. The rats were then sacrificed and the colonic inflammatory scores of rats were measured, including the scores of disease activity index （ DAI）, macroscopic lesions, and histological damages, as well as the activity of myeloperoxidase （MPO）. The expressions of major histocompatibility complex class Ⅱ （ MHC Ⅱ ） and costimulatory molecule CD86 on DCs were determined by flow cytometry. Results Compared with the TNBS group, APS group had significantly decreased scores of DAI （ P = 0. 007 ）, macroscopic lesions （ P = 0. 017）, and histological damages （P = 0. 016）. Moreover, its level of the activity of MPO dropped but without statistical significance （P = 0. 183 ）. TNBS group had significantly higher expressions of MHC Ⅱ and CD86 molecules on DCs than the normal control group （P =0. 005, P =0. 008）, APS group （P =0. 023, P =0. 018）, and 5-ASA group （P = 0. 017, P = 0. 013 ）. Conclusion APS may attenuate TNBS-induced colitis in rats and downregulate the activation of DCs in mesenteric lymph nodes.
出处
《中华临床营养杂志》
CAS
2011年第2期93-97,共5页
Chinese Journal of Clinical Nutrition
关键词
2
4
6-三硝基苯磺酸
结肠炎
黄芪多糖
树突状细胞
炎症性肠病
2,4,6-trinitrobenzenesulfonie acid
Colitis
Astragalus polysaccharides
Dendritic cells
Inflammatory bowel disease
