摘要
目的通过实验证明复方蛇床子灌注液对奶牛乳房炎病原菌的抑杀作用。方法复方蛇床子灌注液为受试药物,6种引起奶牛乳房炎的常见病原菌(金黄色葡萄球菌、大肠埃希菌、无乳链球菌、停乳链球菌、白色念珠菌和热带假丝酵母菌)为实验菌株,采用测定抑菌圈的琼脂平板扩散法和测定最小抑菌浓度(M IC)的药物固体培养基法来评价复方蛇床子灌注液的体外抗菌活性。结果复方蛇床子灌注液对6种供试菌株均有较好的抑菌作用,其中0.1 g/mL的原药液对无乳链球菌和金黄色葡萄球菌表现了强大的抑制作用(M IC值达0.0015 g/mL),抑菌圈直径分别高达33 mm和28 mm。结论复方蛇床子灌注液对奶牛乳房炎6种主要致病菌有较强的抑杀作用,抗菌谱广,药物浓度与抑菌效应呈正相关。
Objective To confirm the inhibitory effect of Fufang Shechuangzi（compound Fructus cnidii） perfusate on pathogenic bacteria of cow mastitis Method Fufang Shechuangzi perfusate was used as a subject drug.Six pathogenic bacteria（Staphylococcusaureus,Escherichia coli,Streptococcus agalactiae,Streptococcus dysgalactiae,Blastomyces albicans and Candida tropicalis） caused cow mastitis were used as experimental strains.The inhibition activities of Fufang Shechuangzi perfusate in vitro were evaluated by the agar plate diffusion method measuring inhibition zone and the solid medium of drug measuring the minimal inhibitory concentration.Result Fufang Shechuangzi perfusate showed better inhibitory activity to these six bacteria.The concentration of 0.1 g/mL Fufang Shechuangzi perfusate had strong inhibitory activity to Streptococcus agalactiae and Staphylococcus aureus（MIC value 0.0015 g/mL） and the sizes of inhibition zone were 33 mm and 28 mm,respectively.Conclusion Fufang Shechuangzi perfusate has strong inhibition activities on six main pathogens of cow mastitis with extensive antibacterial spectrum,and the concentration of the drugs is positively correlated with the inhibition effects.
出处
《中国微生态学杂志》
CAS
CSCD
2011年第4期310-312,共3页
Chinese Journal of Microecology
基金
山东省中青年科学家科研奖励基金资助项目(2008BS07001)
安徽高校省级自然科学研究重点项目(KJ2009A033Z)
关键词
乳房炎
病原菌
中药
抑菌试验
Cow mastitis
Pathogenic bacteria
Traditional Chinese medicine
Inhibition experiment
