摘要
目的探讨微管结合蛋白NuSAP的组织分布特征。方法利用组织斑点杂交和Northern杂交分析NuSAP在多种组织中的分布情况,RT-PCR分析NuSAP在多种肿瘤细胞系中的mRNA水平,Western印迹分析NuSAP在多种肿瘤细胞系中的蛋白水平。结果 NuSAP的组织表达谱分析表明该基因在胸腺、胎肝、骨髓中高表达,提示NuSAP在造血免疫中可能发挥重要功能;在多数肿瘤细胞中均可检测到NuSAP的表达,为分析该基因的功能提供了线索。结论本研究揭示了NuSAP是一种组织特异性的微管结合蛋白,可能在造血/免疫组织中发挥重要功能,研究结果对肿瘤、免疫等疾病的防治具有一定的科学意义。
Objective To analyze the tissue distribution of nucleolar spindle-associated protein NuSAP.Methods Dot blot and Northern blot were both performed to analyze the tissue distribution of NuSAP.RT-PCR was performed to analyze the mRNA level of NuSAP.Total cell lysates were analyzed by Western blotting using rabbit anti-NuSAP antibody.Results NuSAP was highly expressed in the human fetal liver,thymus,bone marrow,indicating that NuSAP might play a role in hematopoietic and immune tissues.NuSAP is expressed in many tumour cell lines in which endogenous NuSAP can be detected.Conclusion NuSAP is an important tissue specific regulator of M phase.
出处
《军事医学科学院院刊》
CSCD
北大核心
2010年第6期509-512,共4页
Bulletin of the Academy of Military Medical Sciences
基金
国家“重大新药创制”科技重大专项关键技术课题(2009ZX09503-002)
重大科学研究计划课题(2010CB912202)
北京市科技新星计划(2007A063)部分资助
