摘要
目的：探讨Ｇｒａｖｅｓ病（ＧＤ）患者异常升高的甲状腺激素与免疫异常之间的因果关系，和抗甲抗腺药治疗ＧＤ期间加用甲状腺素（Ｔ４）的作用。方法：将ＧＤ患者外周血单个核细胞（ＰＢＭＣ）在不同浓度的Ｔ４条件下体外培养６ｄ后，检测Ｔ细胞亚群和培养上清中可溶性白细胞介素２受体（ｓＩＬ２Ｒ）及ＩｇＧ的含量。结果：与正常对照组相比培养后的ＧＤ患者ＰＢＭＣ中ＣＤ８＋Ｔ细胞亚群百分率明显减少（Ｐ＜００１）；培养上清中ｓＩＬ２Ｒ及ＩｇＧ水平显著升高（Ｐ＜００１）；Ｔ４体外对上述指标无明显影响。结论：ＧＤ患者同时具有Ｔ、Ｂ细胞异常；Ｔ４体外对ＧＤ患者的Ｔ、Ｂ细胞功能异常无影响，异常升高的甲状腺激素可能为ＧＤ患者免疫异常的结果而不是导致免疫异常的原因。
Objective: To clarify whether there are T,B
cells abnormalities in Graves' disease(GD) patients and the relationship between those
abnormalities and thyroxine(T4), further investigate the effect of T4 administration on the risk of
relapse of hyperthyroidism during antithyroid drug therapy. Methods: Peripheral blood
mononuclear cells(PBMC) of GD patients and nomal individuals' were incubated at absent or
present of different concentration of T4. The supernatant of cell cultured were used for detecting
IgG and sIL2R and cell pellet were asayed for detecting PBMC surface CD molecules. Results:
In GD patients, the percentage of CD8+ T subset was decreased and CD4+/CD8+ cell ratio and
the level of sIL2R and IgG were increased (P<0.01). T4 had no effect on above parameters(P<0.
05). Conclusion: There are not only B cell but also T cell function abnormalities in GD patients.
The fact that T4 had no effect on T,B function in vitro infer that the excessive thyroxine is
outcome but not the reason of B abnormal in GD patients.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第7期321-323,共3页
Chinese Journal of Immunology
