摘要
目的 探讨肺功能检查专用呼吸过滤器细菌过滤效率的检测方法,并评价其可行性.方法 采用射流雾化器产生带有枯草芽孢杆菌黑色变种标准菌种细菌悬液（浓度为1.5×10^8 cfu/ml）的气溶胶,分别通过3种不同品牌的呼吸过滤器,然后采集过滤器末端的气流进行细菌培养和菌落计数,计算出呼吸过滤器的细菌过滤效率和微生物穿透值.结果 Promed、Microgard和Cosmed 3种过滤器的细菌过滤效率均大于99.99%,各组间差异无统计学意义（P＞0.05）.微生物穿透值分别为（17±9）、（23±12）和（13±8）,3组间及Promed与其余2组间差异无统计学意义（P＞0.05）.结论 通过雾化细菌悬液检测肺功能检查专用呼吸过滤器细菌过滤效率的方法可行.国内产品（Promed）与国内常用进口产品（Microgard和Cosmed）的过滤性能相当,达到呼吸保护装置的过滤要求,适于临床上应用.
Objective To develop an efficiency measurement for bacterial filtration of respiratory filters used in pulmonary function tests to and evaluate its feasibility. Methods An aerosol was generated by jet atomizer using nebulized standard bacterial suspension containing 1.5 × 108 cfu/ml Bacillus subtilis var.niger. The aerosol was driven through three types of commercial respiratory filters. The efferent flow from the filter was collected for bacterial culture and colony count. Bacterial filtration efficiency （BFE） and microbial penetration values （MPV） were computed. Results All the three filters （manufactured by Promed, Cosmed and Microgard） performed a filtration efficiency greater than 99.99% with no significant differences （P〉0.05 ）. The microbial penetration values of the three filters were （ 17 ±9）, （ 23 ± 12） and （ 13 ±8） respectively.There was no significant difference among the groups, or between Promed filters and the other two types （P〉0.05）. Conclusions The use of nebulized bacterial suspension appears feasible to assess the bacterial filtration efficiency of respiratory filters used in pulmonary function test. Homemade filters （Promed） exhibit comparable filtration performance as do imported filters （Microgard and Cosmed） , which meet the requirement for respiratory protective devices and justify their clinical application
出处
《中华生物医学工程杂志》
CAS
2010年第6期561-563,共3页
Chinese Journal of Biomedical Engineering
基金
卫生部面向农村和基层医院适宜卫生技术推广十年百项计划项目（2004-18-01）
关键词
微孔过滤器
呼吸功能试验
芽孢杆菌
枯草
呼吸过滤器
细菌过滤效率
Micropore fileters
Respiratory function tests
Bacillus subtilis
Respiratory filters
Bacterial filtration efficiency
